Jundishapur Journal of Natural Pharmaceutical Products

Published by: Kowsar

Antioxidant and Protective Effects of Angipars™, a New Herbal Drug, on Hydrogen Peroxide-Induced Oxidative Stress in HepG2 and A549 Cell Lines

Elina Asiaei 1 , Melika Farshad 2 , Mohammad Abdollahi 3 , Akram Jamshidzadeh 4 , * , Amin Reza Akbarizadeh 4 and Mohammad Amin Soltanzadeh 1
Authors Information
1 Shiraz University of Medical Sciences, International Branch, Shiraz, Iran
2 Faculty of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
3 Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 1417614411, Iran
4 Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Article information
  • Jundishapur Journal of Natural Pharmaceutical Products: November 2016, 11 (4); e42944
  • Published Online: November 27, 2016
  • Article Type: Research Article
  • Received: October 10, 2016
  • Revised: November 13, 2016
  • Accepted: November 22, 2016
  • DOI: 10.17795/jjnpp-42944

To Cite: Asiaei E, Farshad M, Abdollahi M, Jamshidzadeh A, Akbarizadeh A R, et al. Antioxidant and Protective Effects of Angipars™, a New Herbal Drug, on Hydrogen Peroxide-Induced Oxidative Stress in HepG2 and A549 Cell Lines, Jundishapur J Nat Pharm Prod. 2016 ; 11(4):e42944. doi: 10.17795/jjnpp-42944.

Copyright © 2016, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Kehrer JP. Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol. 1993; 23(1): 21-48[DOI][PubMed]
  • 2. Demple B, Amabile-Cuevas CF. Redox redux: the control of oxidative stress responses. Cell. 1991; 67(5): 837-9[DOI][PubMed]
  • 3. Sies H. Strategies of antioxidant defense. 1994; : 101-7
  • 4. Adly AA. Oxidative stress and disease: an updated review. Res J Immunol. 2010; 3(2): 129-45[DOI]
  • 5. Brandon EF, Bosch TM, Deenen MJ, Levink R, van der Wal E, van Meerveld JB, et al. Validation of in vitro cell models used in drug metabolism and transport studies; genotyping of cytochrome P450, phase II enzymes and drug transporter polymorphisms in the human hepatoma (HepG2), ovarian carcinoma (IGROV-1) and colon carcinoma (CaCo-2, LS180) cell lines. Toxicol Appl Pharmacol. 2006; 211(1): 1-10[DOI][PubMed]
  • 6. Dhalla NS, Temsah RM, Netticadan T. Role of oxidative stress in cardiovascular diseases. J Hypertens. 2000; 18(6): 655-73[PubMed]
  • 7. Halliwell B, Aruoma OI. DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems. FEBS Lett. 1991; 281(1-2): 9-19[DOI][PubMed]
  • 8. Rahimi-Madiseh M, Malekpour-Tehrani A, Bahmani M, Rafieian-Kopaei M. The research and development on the antioxidants in prevention of diabetic complications. Asian Pac J Trop Med. 2016; 9(9): 825-31[DOI][PubMed]
  • 9. Chen W, Jia Z, Pan MH, Babu PVA. Natural Products for the Prevention of Oxidative Stress-Related Diseases: Mechanisms and Strategies. Oxidative Med Cellular Longevity. 2016; 2016
  • 10. Aghel N, Kalantari H, Rezazadeh S. Hepatoprotective effect of Ficus carica leaf extract on mice intoxicated with carbon tetrachloride. Iran J Pharmaceut Res. 2010; : 63-7
  • 11. Kalantari H, Aghel N, Bayati M. Hepatoprotective effect of Morus alba L. in carbon tetrachloride-induced hepatotoxicity in mice. Saudi Pharmaceut J. 2009; 17(1): 90-4
  • 12. Hemmatabadi M, Abdollahi M, Bakhshayeshi S, Heshmat R, Baeeri M, Azimaraghi O. Benefits of Semelil (ANGIPARSTM) on oxidant-antioxidant balance in diabetic patients; A randomized, double-blind placebo controlled clinical trial. DARU J Pharmaceut Sci. 2015; (1): 50-5
  • 13. Farzamfar B, Abdollahi M, Ka'abinejadian S, Heshmat R, Shahhosseiny M, Novitsky Y. Sub-chronic toxicity study of a novel herbal-based formulation (Semelil) on dogs. DARU J Pharmaceut Sci. 2008; 16(1): 15-9
  • 14. Khorram KH, Sadeghi B, Heshmat R, Abdollahi M, Salari P, Farzamfar B. In vivo and in vitro genotoxicity studies of Semelil (ANGIPARS). Daru. 2008; : 16
  • 15. Abdollahi M, Farzamfar B, Salari P, Larijani B, Farhadi M, Madani S. Evaluation of acute and sub-chronic toxicity of Semelil (ANGIPARSTM), a new phytotherapeutic drug for wound healing in rodents. DARU J Pharmaceut Sci. 2008; 16(1): 7-14
  • 16. Plesca-Manea L, Parvu AE, Parvu M, Taamas M, Buia R, Puia M. Effects of Melilotus officinalis on acute inflammation. Phytother Res. 2002; 16(4): 316-9[DOI][PubMed]
  • 17. Alia M, Ramos S, Mateos R, Bravo L, Goya L. Response of the antioxidant defense system to tert-butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2). J Biochem Mol Toxicol. 2005; 19(2): 119-28[DOI][PubMed]
  • 18. Sattayasai J, Chaonapan P, Arkaravichie T, Soi-Ampornkul R, Junnu S, Charoensilp P, et al. Protective effects of mangosteen extract on H2O2-induced cytotoxicity in SK-N-SH cells and scopolamine-induced memory impairment in mice. PLoS One. 2013; 8(12): 85053[DOI][PubMed]
  • 19. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1): 70-6[DOI][PubMed]
  • 20. Chen X, Zhong Z, Xu Z, Chen L, Wang Y. No protective effect of curcumin on hydrogen peroxide-induced cytotoxicity in HepG2 cells. Pharmacol Rep. 2011; 63(3): 724-32[DOI][PubMed]
  • 21. Marabini L, Calo R, Braga PC. Protective effect of erdosteine metabolite I against hydrogen peroxide-induced oxidative DNA-damage in lung epithelial cells. Arzneimittel-Forschung. 2010; 61(12): 700-6
  • 22. Hsu JY, Chu JJ, Chou MC, Chen YW. Dioscorin pre-treatment protects A549 human airway epithelial cells from hydrogen peroxide-induced oxidative stress. Inflammation. 2013; 36(5): 1013-9[DOI][PubMed]
  • 23. Robaszkiewicz A, Balcerczyk A, Bartosz G. Antioxidative and prooxidative effects of quercetin on A549 cells. Cell Biol Int. 2007; 31(10): 1245-50[DOI][PubMed]
  • 24. Jamshidzadeh A, Fereidooni F, Salehi Z, Niknahad H. Hepatoprotective activity of Gundelia tourenfortii. J Ethnopharmacol. 2005; 101(1-3): 233-7[DOI][PubMed]
  • 25. Jamshidzadeh A, Baghban M, Azarpira N, Mohammadi Bardbori A, Niknahad H. Effects of tomato extract on oxidative stress induced toxicity in different organs of rats. Food Chem Toxicol. 2008; 46(12): 3612-5[DOI][PubMed]
  • 26. Jamshidzadeh A, Heidari R, Razmjou M, Karimi F, Moein MR, Farshad O, et al. An in vivo and in vitro investigation on hepatoprotective effects of Pimpinella anisum seed essential oil and extracts against carbon tetrachloride-induced toxicity. Iran J Basic Med Sci. 2015; 18(2): 205-11[PubMed]
  • 27. Al-Sheddi ES, Farshori NN, Al-Oqail MM, Musarrat J, Al-Khedhairy AA, Siddiqui MA. Protective effect of Lepidium sativum seed extract against hydrogen peroxide-induced cytotoxicity and oxidative stress in human liver cells (HepG2). Pharm Biol. 2016; 54(2): 314-21[DOI][PubMed]
  • 28. Kalantaripour T, Asadi-Shekaari M, Basiri M, Najar A. Cerebroprotective effect of date seed extract (Phoenix dactylifera) on focal cerebral ischemia in male rats. J Biol Sci. 2012; 12(3): 180[DOI]
  • 29. Larijani B, Heshmat R, Bahrami A, Delshad H, Mohammad K, Heidarpour R. Effects of intravenous Semelil (ANGIPARSTM) on diabetic foot ulcers healing: A multicenter clinical trial. DARU J Pharmaceut Sci. 2008; 16(1): 35-40
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments