Jundishapur Journal of Natural Pharmaceutical Products

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Assessment of Apoptosis Induction by Methanol Extract of Sea Cucumber in Blastocystis hominis Isolated from Human Samples Using Flow Cytometry and DNA Fragmentation Test

Asma Karamkhani 1 and Jasem Saki 2 , *
Authors Information
1 Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Article information
  • Jundishapur Journal of Natural Pharmaceutical Products: 14 (2); e13959
  • Published Online: May 14, 2019
  • Article Type: Research Article
  • Received: May 23, 2017
  • Revised: January 10, 2019
  • Accepted: February 4, 2018
  • DOI: 10.5812/jjnpp.13959

To Cite: Karamkhani A, Saki J. Assessment of Apoptosis Induction by Methanol Extract of Sea Cucumber in Blastocystis hominis Isolated from Human Samples Using Flow Cytometry and DNA Fragmentation Test, Jundishapur J Nat Pharm Prod. Online ahead of Print ; 14(2):e13959. doi: 10.5812/jjnpp.13959.

Copyright © 2019, Jundishapur Journal of Natural Pharmaceutical Products. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Parija SC, Jeremiah S. Blastocystis: Taxonomy, biology and virulence. Trop Parasitol. 2013;3(1):17-25. doi: 10.4103/2229-5070.113894. [PubMed: 23961437]. [PubMed Central: PMC3745665].
  • 2. Basak S, Rajurkar MN, Mallick SK. Detection of Blastocystis hominis: A controversial human pathogen. Parasitol Res. 2014;113(1):261-5. doi: 10.1007/s00436-013-3652-4. [PubMed: 24169810].
  • 3. Badparva E, Ezatpour B, Mahmoudvand H, Behzadifar M, Behzadifar M, Kheirandish F. Prevalence and genotype analysis of Blastocystis hominis in Iran: A systematic review and meta-analysis. Arch Clin Infect Dis. 2016;12(1). doi: 10.5812/archcid.36648.
  • 4. Khoshnood S, Rafiei A, Saki J, Alizadeh K. Prevalence and genotype characterization of Blastocystis hominis among the baghmalek people in southwestern Iran in 2013 - 2014. Jundishapur J Microbiol. 2015;8(10). e23930. doi: 10.5812/jjm.23930. [PubMed: 26587213]. [PubMed Central: PMC4644266].
  • 5. Wawrzyniak I, Poirier P, Viscogliosi E, Dionigia M, Texier C, Delbac F, et al. Blastocystis, an unrecognized parasite: An overview of pathogenesis and diagnosis. Ther Adv Infect Dis. 2013;1(5):167-78. doi: 10.1177/2049936113504754. [PubMed: 25165551]. [PubMed Central: PMC4040727].
  • 6. Wu Z, Mirza H, Tan KS. Intra-subtype variation in enteroadhesion accounts for differences in epithelial barrier disruption and is associated with metronidazole resistance in Blastocystis subtype-7. PLoS Negl Trop Dis. 2014;8(5). e2885. doi: 10.1371/journal.pntd.0002885. [PubMed: 24851944]. [PubMed Central: PMC4031124].
  • 7. World Health Organization. Guidelines for drinking-water quality: Incorporating first and second addenda to third edition. 1. Geneva: World Health Organization Press; 2008.
  • 8. Hameed DM, Hassanin OM, Zuel-Fakkar NM. Association of Blastocystis hominis genetic subtypes with urticaria. Parasitol Res. 2011;108(3):553-60. doi: 10.1007/s00436-010-2097-2. [PubMed: 20922413].
  • 9. Javaherizadeh H, Khademvatan S, Soltani S, Torabizadeh M, Yousefi E. Distribution of haematological indices among subjects with Blastocystis hominis infection compared to controls. Prz Gastroenterol. 2014;9(1):38-42. doi: 10.5114/pg.2014.40849. [PubMed: 24868297]. [PubMed Central: PMC4027846].
  • 10. Khademvatan S, Masjedizadeh R, Rahim F, Mahbodfar H, Salehi R, Yousefi-Razin E, et al. Blastocystis and irritable bowel syndrome: Frequency and subtypes from Iranian patients. Parasitol Int. 2017;66(2):142-5. doi: 10.1016/j.parint.2017.01.005. [PubMed: 28087441].
  • 11. Kumarasamy V, Kuppusamy UR, Samudi C, Kumar S. Blastocystis sp. subtype 3 triggers higher proliferation of human colorectal cancer cells, HCT116. Parasitol Res. 2013;112(10):3551-5. doi: 10.1007/s00436-013-3538-5. [PubMed: 23933809].
  • 12. Moosavi A, Haghighi A, Mojarad EN, Zayeri F, Alebouyeh M, Khazan H, et al. Genetic variability of Blastocystis sp. isolated from symptomatic and asymptomatic individuals in Iran. Parasitol Res. 2012;111(6):2311-5. doi: 10.1007/s00436-012-3085-5. [PubMed: 22948205].
  • 13. Coyle CM, Varughese J, Weiss LM, Tanowitz HB. Blastocystis: To treat or not to treat. Clin Infect Dis. 2012;54(1):105-10. doi: 10.1093/cid/cir810. [PubMed: 22075794].
  • 14. Stensvold CR, Smith HV, Nagel R, Olsen KE, Traub RJ. Eradication of Blastocystis carriage with antimicrobials: Reality or delusion? J Clin Gastroenterol. 2010;44(2):85-90. doi: 10.1097/MCG.0b013e3181bb86ba. [PubMed: 19834337].
  • 15. Mirza H, Teo JD, Upcroft J, Tan KS. A rapid, high-throughput viability assay for Blastocystis spp. reveals metronidazole resistance and extensive subtype-dependent variations in drug susceptibilities. Antimicrob Agents Chemother. 2011;55(2):637-48. doi: 10.1128/AAC.00900-10. [PubMed: 21098237]. [PubMed Central: PMC3028762].
  • 16. Lianet M. Editorial (thematic issue: Development of natural products as anti-parasitic agents). Current Clinical Pharmacology. 2014;9(3):181-6. doi: 10.2174/157488470903140806112509.
  • 17. Frasson AP, dos Santos O, Duarte M, da Silva Trentin D, Giordani RB, da Silva AG, et al. First report of anti-Trichomonas vaginalis activity of the medicinal plant Polygala decumbens from the Brazilian semi-arid region, Caatinga. Parasitol Res. 2012;110(6):2581-7. doi: 10.1007/s00436-011-2787-4. [PubMed: 22205352].
  • 18. Kone WM, Vargas M, Keiser J. Anthelmintic activity of medicinal plants used in Cote d'Ivoire for treating parasitic diseases. Parasitol Res. 2012;110(6):2351-62. doi: 10.1007/s00436-011-2771-z. [PubMed: 22200959].
  • 19. Arnold PW, Birtles A. Soft-sediment marine invertebrates of southeast asia and australia: A guide to identification. Australian Institute of Marine Science; 1989.
  • 20. Bordbar S, Anwar F, Saari N. High-value components and bioactives from sea cucumbers for functional foods--a review. Mar Drugs. 2011;9(10):1761-805. doi: 10.3390/md9101761. [PubMed: 22072996]. [PubMed Central: PMC3210605].
  • 21. Aminin DL, Chaykina EL, Agafonova IG, Avilov SA, Kalinin VI, Stonik VA. Antitumor activity of the immunomodulatory lead Cumaside. Int Immunopharmacol. 2010;10(6):648-54. doi: 10.1016/j.intimp.2010.03.003. [PubMed: 20227525].
  • 22. Aydin M, Sevgili H, Tufan B, Emre Y, Kose S. Proximate composition and fatty acid profile of three different fresh and dried commercial sea cucumbers from Turkey. Int J Food Sci Tech. 2011;46(3):500-8. doi: 10.1111/j.1365-2621.2010.02512.x.
  • 23. Chen S, Li G, Yin L, Huang W, Dong P, Xu J, et al. Identification of eight species of sea cucumber chondroitin sulfates by high temperature 1H NMR. J Instrum Anal. 2010;29(8).
  • 24. Hamaguchi P, Geirsdottir M, Vrac A, Kristinsson H, Sveinsdottir H, Fridjonsson O, et al. In vitro antioxidant and antihypertensive properties of Icelandic sea cucumber (Cucumaria frondosa). Institute of Food Technologists Annual Meeting. Chicago, IL. 2010.
  • 25. Janakiram NB, Mohammed A, Zhang Y, Choi CI, Woodward C, Collin P, et al. Chemopreventive effects of Frondanol A5, a Cucumaria frondosa extract, against rat colon carcinogenesis and inhibition of human colon cancer cell growth. Cancer Prev Res (Phila). 2010;3(1):82-91. doi: 10.1158/1940-6207.CAPR-09-0112. [PubMed: 20051375].
  • 26. Singh N, Kumar R, Gupta S, Dube A, Lakshmi V. Antileishmanial activity in vitro and in vivo of constituents of sea cucumber Actinopyga lecanora. Parasitol Res. 2008;103(2):351-4. doi: 10.1007/s00436-008-0979-3. [PubMed: 18452039].
  • 27. Wang J, Wang Y, Tang Q, Wang Y, Chang Y, Zhao Q, et al. Antioxidation activities of low-molecular-weight gelatin hydrolysate isolated from the sea cucumber Stichopus japonicus. J Ocean Univ China. 2010;9(1):94-8. doi: 10.1007/s11802-010-0094-9.
  • 28. Yuan WH, Yi YH, Tang HF, Liu BS, Wang ZL, Sun GQ, et al. Antifungal triterpene glycosides from the sea cucumber Bohadschia marmorata. Planta Med. 2009;75(2):168-73. doi: 10.1055/s-0028-1088348. [PubMed: 19096993].
  • 29. Zancan P, Mourao PA. Venous and arterial thrombosis in rat models: Dissociation of the antithrombotic effects of glycosaminoglycans. Blood Coagul Fibrinolysis. 2004;15(1):45-54. [PubMed: 15166943].
  • 30. Huerta S, Goulet EJ, Huerta-Yepez S, Livingston EH. Screening and detection of apoptosis. J Surg Res. 2007;139(1):143-56. doi: 10.1016/j.jss.2006.07.034. [PubMed: 17257621].
  • 31. Nasirudeen AM. Cell death and human intestinal protozoa: A brief overview. Curr Issues Intest Microbiol. 2005;6(2):77-82. [PubMed: 16107040].
  • 32. Yin J, Howe J, Tan KS. Staurosporine-induced programmed cell death in Blastocystis occurs independently of caspases and cathepsins and is augmented by calpain inhibition. Microbiology. 2010;156(Pt 5):1284-93. doi: 10.1099/mic.0.034025-0. [PubMed: 20056704].
  • 33. Khademvatan S, Eskandari A, Saki J, Foroutan-Rad M. Cytotoxic activity of holothuria leucospilota extract against leishmania infantum in vitro. Adv Pharmacol Sci. 2016;2016:8195381. doi: 10.1155/2016/8195381. [PubMed: 27022392]. [PubMed Central: PMC4789066].
  • 34. Zhang X, Qiao JY, Zhou XJ, Yao FR, Wei ZC. Morphology and reproductive mode of Blastocystis hominis in diarrhea and in vitro. Parasitol Res. 2007;101(1):43-51. doi: 10.1007/s00436-006-0439-x. [PubMed: 17216486].
  • 35. El Deeb HK, Al Khadrawy FM, Abd El-Hameid AK. Inhibitory effect of Ferula asafoetida L. (Umbelliferae) on Blastocystis sp. subtype 3 growth in vitro. Parasitol Res. 2012;111(3):1213-21. doi: 10.1007/s00436-012-2955-1. [PubMed: 22584378].
  • 36. Li XH, Deng T, Li J. [Experimental observation of blastocystis hominis cultured in modified RPMI 1640]. J Gannan Med Univ. 2013;4(2). China.
  • 37. Carmichael J, Mitchell JB, DeGraff WG, Gamson J, Gazdar AF, Johnson BE, et al. Chemosensitivity testing of human lung cancer cell lines using the MTT assay. Br J Cancer. 1988;57(6):540-7. [PubMed: 2841961]. [PubMed Central: PMC2246465].
  • 38. Song M, Park DK, Cho M, Park HJ. Anti-inflammatory and anti-allergic activities of sea cucumber (Stichopus japonicus) extract. Food Sci Biotech. 2013;22(6):1661-6. doi: 10.1007/s10068-013-0264-9.
  • 39. Nasirudeen AM, Hian YE, Singh M, Tan KS. Metronidazole induces programmed cell death in the protozoan parasite Blastocystis hominis. Microbiology. 2004;150(Pt 1):33-43. doi: 10.1099/mic.0.26496-0. [PubMed: 14702395].
  • 40. Fumarola L, Spinelli R, Brandonisio O. In vitro assays for evaluation of drug activity against Leishmania spp. Res Microbiol. 2004;155(4):224-30. doi: 10.1016/j.resmic.2004.01.001. [PubMed: 15142618].
  • 41. Foroutan-Rad M, Khademvatan S, Saki J, Hashemitabar M. Holothuria leucospilota extract induces apoptosis in leishmania major promastigotes. Iran J Parasitol. 2016;11(3):339-49. [PubMed: 28127339]. [PubMed Central: PMC5256050].
  • 42. Lu Y, Zhang BY, Dong Q, Wang BL, Sun XB. The effects of Stichopus japonicus acid mucopolysaccharide on the apoptosis of the human hepatocellular carcinoma cell line HepG2. Am J Med Sci. 2010;339(2):141-4. doi: 10.1097/MAJ.0b013e3181c20d01. [PubMed: 20051817].
  • 43. Gowda NM, Goswami U, Khan MI. Purification and characterization of a T-antigen specific lectin from the coelomic fluid of a marine invertebrate, sea cucumber (Holothuria scabra). Fish Shellfish Immunol. 2008;24(4):450-8. doi: 10.1016/j.fsi.2008.01.002. [PubMed: 18282768].
  • 44. Lu Y, Wang BL. The research progress of antitumorous effectiveness of Stichopus japonicus acid mucopolysaccharide in north of China. Am J Med Sci. 2009;337(3):195-8. doi: 10.1097/MAJ.0b013e318182ee45. [PubMed: 19174693].
  • 45. Wu M, Xu S, Zhao J, Kang H, Ding H. Free-radical depolymerization of glycosaminoglycan from sea cucumber Thelenata ananas by hydrogen peroxide and copper ions. Carbohyd Polym. 2010;80(4):1116-24. doi: 10.1016/j.carbpol.2010.01.032.
  • 46. Zhang Y, Song S, Song D, Liang H, Wang W, Ji A. Proliferative effects on neural stem/progenitor cells of a sulfated polysaccharide purified from the sea cucumber Stichopus japonicus. J Biosci Bioeng. 2010;109(1):67-72. doi: 10.1016/j.jbiosc.2009.07.010. [PubMed: 20129085].
  • 47. Zhao Q, Wang JF, Xue Y, Wang Y, Gao S, Lei M, et al. Comparative study on the bioactive components and immune function of three species of sea cucumber. J Fishery Sci China. 2008;1(22).
  • 48. Ogushi M, Yoshie-Stark Y, Suzuki T. Cytostatic activity of hot water extracts from the sea cucumber in caco-2. Food Sci Tech Res. 2005;11(2):202-6. doi: 10.3136/fstr.11.202.
  • 49. Adibpour N, Nasr F, Nematpour F, Shakouri A, Ameri A. Antibacterial and antifungal activity of holothuria leucospilota isolated from Persian Gulf and Oman sea. Jundishapur J Microbiol. 2014;7(1). e8708. doi: 10.5812/jjm.8708. [PubMed: 25147657]. [PubMed Central: PMC4138674].
  • 50. Ahmed MAF, Ismail KA, Ahmed SAEG, Ibrahim AN, Gohar YM. In vitro activity of curcumin and silver nanoparticles against Blastocystis hominis. Infect Dis Clin Prac. 2015;23(3):135-40. doi: 10.1097/ipc.0000000000000242.
  • 51. Bremer Christensen C, Soelberg J, Stensvold CR, Jager AK. Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis. J Ethnopharmacol. 2015;174:569-75. doi: 10.1016/j.jep.2015.03.006. [PubMed: 25773490].

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