Jundishapur Journal of Natural Pharmaceutical Products

Published by: Kowsar

Cloning, Transformation and Expression of Human Interferon α2b Gene in Tobacco Plant (Nicotiana tabacum cv. xanthi)

Shahrzad Ahangarzadeh 1 , 2 , Mohammad Hosein Daneshvar 1 , Hamid Rajabi-Memari 3 , * , Hamid Galehdari 4 and Khalil Alamisaied 1
Authors Information
1 Department of Agricultural Biotechnology, University of Agriculture and Natural Resources, Ahvaz, IR Iran
2 Department of Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
3 Department of Agronomy and Plant Breeding, Shahid Chamran University, Ahvaz, IR Iran
4 Department of Genetics, Shahid Chamran University, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Natural Pharmaceutical Products: August 25, 2012, 7 (3); 111-116
  • Published Online: August 27, 2012
  • Article Type: Research Article
  • Received: December 2, 2011
  • Revised: February 26, 2012
  • Accepted: June 6, 2012
  • DOI: 10.17795/jjnpp-3678

To Cite: Ahangarzadeh S, Daneshvar M H, Rajabi-Memari H, Galehdari H, Alamisaied K. Cloning, Transformation and Expression of Human Interferon α2b Gene in Tobacco Plant (Nicotiana tabacum cv. xanthi), Jundishapur J Nat Pharm Prod. 2012 ; 7(3):111-116. doi: 10.17795/jjnpp-3678.

Abstract
Copyright © 2012, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Schillberg S, Emans N, Fischer R. Antibody molecular farming in plants and plant cells. Phytochem Rev. 2002; 1(1): 45-54[DOI]
  • 2. Fischer R, Schillberg S. Molecular farming: Plant-made pH pharmaceuticals and technical proteins. 2004;
  • 3. Chelbi-Alix MK, Wietzerbin J. Interferon, a growing cytokine family: 50 years of interferon research. Biochimie. 2007; 89(6-7): 713-8[DOI][PubMed]
  • 4. Kurane I, Meager A, Ennis FA. Induction of interferon alpha and gamma from human lymphocytes by dengue virus-infected cells. J Gen Virol. 1986; 67: 1653-61[DOI][PubMed]
  • 5. Rubinstein M, Levy WP, Moschera JA, Lai CY, Hershberg RD, Bartlett RT, et al. Human leukocyte interferon: isolation and characterization of several molecular forms. Arch Biochem Biophys. 1981; 210(1): 307-18[DOI]
  • 6. Lee SH, Kelley S, Chiu H, Stebbing N. Stimulation of natural killer cell activity and inhibition of proliferation of various leukemic cells by purified human leukocyte interferon subtypes. Cancer Res. 1982; 42(4): 1312-6[PubMed]
  • 7. Strander H, Mogensen KE, Cantell K. Production of human lymphoblastoid interferon. J Clin Microbiol. 1975; 1(1): 116-7[PubMed]
  • 8. Attallah AM, Fleisher TA, Hu R, Abdel-Gaffar H, Ibrahim S, Metwali A, et al. Immunological activities of recombinant interferon-alpha 2 and its A fragment. J Interferon Res. 1987; 7(2): 223-9[DOI][PubMed]
  • 9. Sen GC, Lengyel P. The interferon system. A bird’s eye view of its biochemistry. J Biol Chem. 1992; 267(8): 5017-20[PubMed]
  • 10. Maeda S, McCandliss R, Gross M, Sloma A, Familletti PC, Tabor JM, et al. Construction and identification of bacterial plasmids containing nucleotide sequence for human leukocyte interferon. Proc Natl Acad Sci U S A. 1980; 77(12): 7010-3[DOI]
  • 11. Lund P, Dunsmuir P. A plant signal sequence enhances the secretion of bacterial ChiA in transgenic tobacco. Plant Mol Biol. 1992; 18(1): 47-53[DOI][PubMed]
  • 12. Bergmann L. Growth and division of single cells of higher plants in vitro. J Gen Physiol. 1960; 43(4): 841-51[DOI][PubMed]
  • 13. De La Riva GA, González-Cabrera J, Vázquez-Padrón R, Ayra-Pardo C. Agrobacterium tumefaciens: a natural tool for plant transformation. Electron J Biotechn. 1998; 1(3): 24-5
  • 14. Sambrook J, Russell DW. Molecular cloning: a laboratory manual. 2001;
  • 15. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol plantarum. 1962; 15(3): 473-97[DOI]
  • 16. Dellaporta SL, Wood J, Hicks JB. A plant DNA minipreparation: version II. Plant Molec Biol. 1983; 1(4): 19-21[DOI]
  • 17. Schillberg S, Fischer R, Emans N. ‘Molecular farming’ of antibodies in plants. Naturwissenschaften. 2003; 90(4): 145-55[PubMed]
  • 18. Kumar G, Ganapathi T, Bapat V. Production of hepatitis B surface antigen in recombinant plant systems: an update. Biotechnol Prog. 2007; 23(3): 532-9[DOI][PubMed]
  • 19. Rajabi Memari H, Ramanan RN, Ariff AB. Comparison of expression systems for the production of human interferon-α2b. Cent Eur J Biol. 2010; 5(4): 446-55[DOI]
  • 20. Leelavathi S, Reddy VS. Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors. Mol Breeding. 2003; 11(1): 49-58[DOI]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments