Jundishapur Journal of Natural Pharmaceutical Products

Published by: Kowsar

Anxiolytic and Hypnotic Effects of Aqueous and Ethanolic Extracts of Aerial Parts of Echium italicum L. in Mice

Hossein Hosseinzadeh 1 , * , Shabnam Shahandeh 1 and Shabnam Shahsavand 1
Authors Information
1 Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran
Article information
  • Jundishapur Journal of Natural Pharmaceutical Products: May 28, 2012, 7 (2); 71-79
  • Published Online: May 28, 2012
  • Article Type: Research Article
  • Received: November 20, 2011
  • Revised: February 15, 2012
  • Accepted: February 22, 2012
  • DOI: 10.17795/jjnpp-4589

To Cite: Hosseinzadeh H, Shahandeh S, Shahsavand S. Anxiolytic and Hypnotic Effects of Aqueous and Ethanolic Extracts of Aerial Parts of Echium italicum L. in Mice, Jundishapur J Nat Pharm Prod. 2012 ; 7(2):71-79. doi: 10.17795/jjnpp-4589.

Copyright © 2012, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Rechinger KH. Flora Iranica: Ceratophyllaceae. 1966;
  • 2. Papageorgiou V, Assimopoulou A, Kyriacou G. Determination of naturally occurring hydroxynaphthoquinone polymers by size-exclusion chromatography. Chromatographia. 2002; 55(7): 423-30[DOI]
  • 3. Papageorgiou V, Assimopoulou A, Samanidou V, Papadoyannis I. Analytical methods for the determination of alkannins and shikonins. Curr Org Chem. 2006; 10(5): 583-622[DOI]
  • 4. Papageorgiou VP, Assimopoulou AN, Couladouros EA, Hepworth D, Nicolaou K. The chemistry and biology of alkannin, shikonin, and related naphthazarin natural products. Angew Chem Int Edit. 1999; 38(3): 270-301
  • 5. Papageorgiou VP, Assimopoulou AN, Samanidou V, Papadoyannis I. Recent advances in chemistry, biology and biotechnology of alkannins and shikonins. Curr Org Chem. 2006; 10(16): 2123-42[DOI]
  • 6. Papagiannis J, Chatzis A, Sarris G. Giant right atrial aneurysm: a case report. Int J Cardiol. 2008; 129(1)-6[DOI][PubMed]
  • 7. Shen CC, Syu WJ, Li SY, Lin CH, Lee GH, Sun CM. Antimicrobial activities of naphthazarins from Arnebia euchroma. J Nat Prod. 2002; 65(12): 1857-62[DOI][PubMed]
  • 8. Chen X, Yang L, Zhang N, Turpin JA, Buckheit RW, Osterling C, et al. Shikonin, a component of chinese herbal medicine, inhibits chemokine receptor function and suppresses human immunodeficiency virus type 1. Antimicrob Agents Chemother. 2003; 47(9): 2810-6[DOI][PubMed]
  • 9. Yoon Y, Kim YO, Lim NY, Jeon WK, Sung HJ. Shikonin, an ingredient of Lithospermum erythrorhizon induced apoptosis in HL60 human premyelocytic leukemia cell line. Planta Med. 1999; 65(6): 532-5[DOI][PubMed]
  • 10. Singh F, Gao D , Lebwohl MG, Wei H. Shikonin modulates cell proliferation by inhibiting epidermal growth factor receptor signaling in human epidermoid carcinoma cells. Cancer Lett. 2003; 200(2): 115-21[DOI]
  • 11. Chen X, Oppenheim J, Howard OM. Shikonin, a component of antiinflammatory Chinese herbal medicine, selectively blocks chemokine binding to CC chemokine receptor-1. Int Immunopharmacol. 2001; 1(2): 229-36[DOI]
  • 12. Kourounakis AP, Assimopoulou AN, Papageorgiou VP, Gavalas A, Kourounakis PN. Alkannin and shikonin: effect on free radical processes and on inflammation - a preliminary pharmacochemical investigation. Arch Pharm (Weinheim). 2002; 335(6): 262-6
  • 13. Staniforth V, Wang SY, Shyur LF, Yang NS. Shikonins, phytocompounds from Lithospermum erythrorhizon, inhibit the transcriptional activation of human tumor necrosis factor alpha promoter in vivo. J Biol Chem. 2004; 279(7): 5877-85[DOI][PubMed]
  • 14. Assimopoulou A, Boskou D, Papageorgiou V. Antioxidant activities of alkannin, shikonin and Alkanna tinctoria root extracts in oil substrates. Food chem. 2004; 87(3): 433-38[DOI]
  • 15. Cho MH, Paik YS, Hahn TR. Physical stability of shikonin derivatives from the roots of Lithospermum erythrorhizon cultivated in Korea. J Agric Food Chem. 1999; 47(10): 4117-20[DOI][PubMed]
  • 16. Amin G. Popular medicinal plants of Iran. 1991;
  • 17. Rabbani M, Sajjadi SE, Vaseghi G, Jafarian A. Anxiolytic effects of Echium amoenum on the elevated plus-maze model of anxiety in mice. Fitoterapia. 2004; 75(5): 457-64[DOI][PubMed]
  • 18. Fazly Bazzaz B, Haririzadeh G, Imami S, Rashed M. Survey of Iranian plants for alkaloids, flavonoids, saponins, and tannins [Khorasan Province]. Pharm Bio. 1997; 35(1): 17-30[DOI]
  • 19. Teo SK, Stirling DI, Thomas SD, Khetani VD. Neurobehavioral effects of racemic threo-methylphenidate and its D and L enantiomers in rats. Pharmacol Biochem Behav. 2003; 74(3): 747-54[DOI]
  • 20. Hosseinzadeh H, Nourbakhsh M. Effect of Rosmarinus officinalis L. aerial parts extract on morphine withdrawal syndrome in mice. Phytother Res. 2003; 17(8): 938-41[DOI][PubMed]
  • 21. Hosseinzadeh H, Noraei NB. Anxiolytic and hypnotic effect of Crocus sativus aqueous extract and its constituents, crocin and safranal, in mice. Phytother Res. 2009; 23(6): 768-74[DOI][PubMed]
  • 22. Pellow S, Chopin P, File SE, Briley M. Validation of open:closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods. 1985; 14(3): 149-67[DOI]
  • 23. Kang TH, Jeong SJ, Kim NY, Higuchi R, Kim YC. Sedative activity of two flavonol glycosides isolated from the flowers of Albizzia julibrissin Durazz. J Ethnopharmacol. 2000; 71(1-2): 321-3[DOI]
  • 24. American Psychiatric Association. Diagnostic and statistical manual of mental disorders. 2000;
  • 25. Nutt DJ, Malizia AL. New insights into the role of the GABA(A)-benzodiazepine receptor in psychiatric disorder. Br J Psychiatry. 2001; 179: 390-6[DOI][PubMed]
  • 26. Tyrer P, Baldwin D. Generalised anxiety disorder. Lancet. 2006; 368(9553): 2156-66[DOI]
  • 27. Sarris J. Herbal medicines in the treatment of psychiatric disorders: a systematic review. Phytother Res. 2007; 21(8): 703-16[DOI][PubMed]
  • 28. Sarris J, Kavanagh DJ. Kava and St. John's Wort: current evidence for use in mood and anxiety disorders. J Altern Complement Med. 2009; 15(8): 827-36[DOI][PubMed]
  • 29. Awad R, Levac D, Cybulska P, Merali Z, Trudeau VL, Arnason JT. Effects of traditionally used anxiolytic botanicals on enzymes of the gamma-aminobutyric acid (GABA) system. Can J Physiol Pharmacol. 2007; 85(9): 933-42[DOI][PubMed]
  • 30. Spinella M. Herbal Medicines and Epilepsy: The Potential for Benefit and Adverse Effects. Epilepsy Behav. 2001; 2(6): 524-32[DOI][PubMed]
  • 31. Baldwin DS, Polkinghorn C. Evidence-based pharmacotherapy of Generalized Anxiety Disorder. Int J Neuropsychopharmacol. 2005; 8(2): 293-302[DOI][PubMed]
  • 32. Medina JH, Viola H, Wolfman C, Marder M, Wasowski C, Calvo D, et al. Overview--flavonoids: a new family of benzodiazepine receptor ligands. Neurochem Res. 1997; 22(4): 419-25[DOI][PubMed]
  • 33. Paladini AC, Marder M, Viola H, Wolfman C, Wasowski C, Medina JH. Flavonoids and the central nervous system: from forgotten factors to potent anxiolytic compounds. J Pharm Pharmacol. 1999; 51(5): 519-26[DOI][PubMed]
  • 34. Wolfman C, Viola H, Marder M, Wasowski C, Ardenghi P, Izquierdo I, et al. Anxioselective properties of 6,3'-dinitroflavone, a high-affinity benzodiazepine receptor ligand. Eur J Pharmacol. 1996; 318(1): 23-30[DOI]
  • 35. Liao JF, Hung WY, Chen CF. Anxiolytic-like effects of baicalein and baicalin in the Vogel conflict test in mice. Eur J Pharmacol. 2003; 464(2-3): 141-6[DOI]
  • 36. Wolfman C, Viola H, Paladini A, Dajas F, Medina JH. Possible anxiolytic effects of chrysin, a central benzodiazepine receptor ligand isolated from Passiflora coerulea. Pharmacol Biochem Behav. 1994; 47(1): 1-4[DOI]
  • 37. Hui KM, Huen MS, Wang HY, Zheng H, Sigel E, Baur R, et al. Anxiolytic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi. Biochem Pharmacol. 2002; 64(9): 1415-24[DOI]
  • 38. Chojkier M. Hepatic sinusoidal-obstruction syndrome: toxicity of pyrrolizidine alkaloids. J Hepatol. 2003; 39(3): 437-46[DOI]
  • 39. Mei N, Guo L, Fu PP, Heflich RH, Chen T. Mutagenicity of comfrey (Symphytum Officinale) in rat liver. Br J Cancer. 2005; 92(5): 873-5[DOI][PubMed]
  • 40. Wang YP, Yan J, Fu PP, Chou MW. Human liver microsomal reduction of pyrrolizidine alkaloid N-oxides to form the corresponding carcinogenic parent alkaloid. Toxicol Lett. 2005; 155(3): 411-20[DOI][PubMed]
  • 41. Seaman JT, Dixon RJ. Investigations into the toxicity of Echium plantagineum in sheep. 2. Pen feeding experiments. Aust Vet J. 1989; 66(9): 286-92[DOI][PubMed]
  • 42. Albreht A, Vovk I, Simonovska B, Srbinoska M. Identification of shikonin and its ester derivatives from the roots of Echium italicum L. J Chromatogr A. 2009; 1216(15): 3156-62[DOI][PubMed]
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