Jundishapur Journal of Natural Pharmaceutical Products

Published by: Kowsar

Determination of the Mutagenicity Potential of Sankol Herbal Medicine by Single Cell Gel Electrophoresis in Rat Hepatocytes in Comparison With H2O2

Heibatullah Kalantari 1 , Mohsen Rezaei 1 , Masoud Mahdavinia 1 , Rashid Jahangirnejad 1 and Golnaz Varnaseri 1 , *
Authors Information
1 School of Pharmacy, Medicinal plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Natural Pharmaceutical Products: August 25, 2012, 7 (3); 123-126
  • Published Online: August 25, 2012
  • Article Type: Research Article
  • Received: May 7, 2012
  • Revised: June 17, 2012
  • Accepted: June 27, 2012
  • DOI: 10.17795/jjnpp-5480

To Cite: Kalantari H, Rezaei M, Mahdavinia M, Jahangirnejad R, Varnaseri G. Determination of the Mutagenicity Potential of Sankol Herbal Medicine by Single Cell Gel Electrophoresis in Rat Hepatocytes in Comparison With H2O2, Jundishapur J Nat Pharm Prod. 2012 ; 7(3):123-126. doi: 10.17795/jjnpp-5480.

Copyright © 2012, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Ahn HS, Jeon TI, Lee JY, Hwang SG, Lim Y, Park DK. Antioxidative activity of persimmon and grape seed extract: in vitro and in vivo. Nutr Res. 2002; 22(11): 1265-73[DOI]
  • 2. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001; 109 Suppl 1: 69-75[DOI][PubMed]
  • 3. Higashimoto M, Purintrapiban J, Kataoka K, Kinouchi T, Vinitketkumnuen U, Akimoto S, et al. Mutagenicity and antimutagenicity of extracts of three spices and a medicinal plant in Thailand. Mutat Res. 1993; 303(3): 135-42[DOI]
  • 4. Kassie F, Parzefall W, Musk S, Johnson I, Lamprecht G, Sontag G, et al. Genotoxic effects of crude juices from Brassica vegetables and juices and extracts from phytopharmaceutical preparations and spices of cruciferous plants origin in bacterial and mammalian cells. Chem Biol Interact. 1996; 102(1): 1-16[DOI]
  • 5. Liu RH. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr. 2003; 78(3 Suppl): 517S-20S[PubMed]
  • 6. McGaw LJ, Jager AK, van Staden J. Antibacterial, anthelmintic and anti-amoebic activity in South African medicinal plants. J Ethnopharmacol. 2000; 72(1-2): 247-63[DOI]
  • 7. Rabe T, Van Staden J. Screening of Plectranthus species for antibacterial activity. S Afr J Botany. 1998; 64(1): 62-5
  • 8. Schimmer O, Hafele F, Kruger A. The mutagenic potencies of plant extracts containing quercetin in Salmonella typhimurium TA98 and TA100. Mutat Res. 1988; 206(2): 201-8[DOI]
  • 9. Schimmer O, Kruger A, Paulini H, Haefele F. An evaluation of 55 commercial plant extracts in the Ames mutagenicity test. Pharmazie. 1994; 49(6): 448-51[PubMed]
  • 10. Nature and modern pharmaceutical technology. Sankol
  • 11. Rubinstein LV, Shoemaker RH, Paull KD, Simon RM, Tosini S, Skehan P, et al .. Comparison of in vitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines. J Natl Cancer Inst. 1990; 82(13): 1113-8[DOI]
  • 12. Moneef MA, Sherwood BT, Bowman KJ, Kockelbergh RC, Symonds RP, Steward WP, et al. Measurements using the alkaline comet assay predict bladder cancer cell radiosensitivity. Br J Cancer. 2003; 89(12): 2271-6[DOI][PubMed]
  • 13. Berry MN, Phillips JW. The isolated hepatocyte preparation: 30 years on. Biochem Soc Trans. 2000; 28(2): 131-5[PubMed]
  • 14. Speit G, Hartmann A. The comet assay (single-cell gel test). A sensitive genotoxicity test for the detection of DNA damage and repair. Methods Mol Biol. 1999; 113: 203-12[DOI]
  • 15. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1): 184-91[DOI]
  • 16. Jalali M, Hatami A, Kalantari H, Kalantar E. Mutagenicity Assessment of Two Herbal Medicines, Urtan and Carmint in Human Leukocytes by Single Cell Gel Electrophoresis. Saudi Pharm J. 2006; 14(2): 129
  • 17. Junqueira APF, Perazzo FF, Souza GHB, Maistro EL. Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo ma mmalian cell system. Genet Mol Biol. 2007; 30: 656-63[DOI]
  • 18. Kalantari H, Jalali M, Moein E. In Vitro Evaluation of Mutagenic Effect of Vitagnus and Shirafza in Human Leukocytes by Single Cell Gel Electrophoresis. Jundushapur J Nat Pharm Prod. 2007; 1(1): 26-31
  • 19. Hartmann A, Speit G. The contribution of cytotoxicity to DNA-effects in the single cell gel test (comet assay). Toxicol Lett. 1997; 90(2-3): 183-8[DOI]
  • 20. Kalantari H, Panahi M, Izadinajafabadi A, Mohammadi E. Genotoxicity Determinations of Coriander Drop and Extract of Coriander Sativum in Cultured Fibroblast of Rat Embryo by Comet Assay. Saudi Pharm J. 2008; 16(1): 85
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