Jundishapur Journal of Natural Pharmaceutical Products

Published by: Kowsar

Determination of the Mutagenicity Potential of Dillsun Herbal Medicine by Single Cell Gel Electrophoresis in Rat Hepatocytes

Heibatullah Kalantari 1 , Mohsen Rezaei 1 , Maryam Salehcheh 1 , Mehrnoosh Moosavi 1 and Golnaz Varnaseri 1 , *
Authors Information
1 Department of Pharmacology and Toxicology, Medicinal Plants and Natural Products Research Center, School of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Natural Pharmaceutical Products: June 10, 2013, 8 (2); 55-59
  • Published Online: May 4, 2013
  • Article Type: Research Article
  • Received: December 1, 2012
  • Revised: January 21, 2013
  • Accepted: February 4, 2013
  • DOI: 10.17795/jjnpp-9156

To Cite: Kalantari H, Rezaei M, Salehcheh M, Moosavi M, Varnaseri G. Determination of the Mutagenicity Potential of Dillsun Herbal Medicine by Single Cell Gel Electrophoresis in Rat Hepatocytes, Jundishapur J Nat Pharm Prod. 2013 ; 8(2):55-59. doi: 10.17795/jjnpp-9156.

Abstract
Copyright © 2013, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Kunle OF, Egharevba HO, Ahmadu PO. Standardization of herbal medicines-A review. Int J Biodivers Conserv. 2012; 4(3): 101-12
  • 2. Van Wyk BE, van Oudtshoorn B, Gericke N. Medicinal Plants of South Africa. 1997;
  • 3. McGaw LJ, Jager AK, van Staden J. Antibacterial, anthelmintic and anti-amoebic activity in South African medicinal plants. J Ethnopharmacol. 2000; 72(1-2): 247-63[PubMed]
  • 4. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001; 109 Suppl 1: 69-75[PubMed]
  • 5. Schimmer O, Hafele F, Kruger A. The mutagenic potencies of plant extracts containing quercetin in Salmonella typhimurium TA98 and TA100. Mutat Res. 1988; 206(2): 201-8[PubMed]
  • 6. van der Lelie D, Regniers L, Borremans B, Provoost A, Verschaeve L. The VITOTOX test, an SOS bioluminescence Salmonella typhimurium test to measure genotoxicity kinetics. Mutat Res. 1997; 389(2-3): 279-90[PubMed]
  • 7. Garaj-Vrhovac V, Zeljezic D. Comet assay in the assessment of the human genome damage induced by γ-radiation in vitro. Radiol Oncol. 2004; 38(1): 43-7
  • 8. Wong VWC, Szeto YT, Collins AR, Benzie IFF. THE COMET ASSAY: a biomonitoring tool for nutraceutical research. Curr Top in Nutr Res. 2005; 3(1): 1-14
  • 9. Dillsun Oral Drop.
  • 10. Vian L, Yusuf A, Guyomard C, Cano JP. The Liverbeads as a tool for the comet assay. Mutat Res. 2002; 519(1-2): 163-70[PubMed]
  • 11. Rubinstein LV, Shoemaker RH, Paull KD, Simon RM, Tosini S, Skehan P, et al. Comparison of in vitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines. J Natl Cancer Inst. 1990; 82(13): 1113-8[PubMed]
  • 12. Moneef MA, Sherwood BT, Bowman KJ, Kockelbergh RC, Symonds RP, Steward WP, et al. Measurements using the alkaline comet assay predict bladder cancer cell radiosensitivity. Br J Cancer. 2003; 89(12): 2271-6[DOI][PubMed]
  • 13. Speit G, Hartmann A. The comet assay (single-cell gel test). A sensitive genotoxicity test for the detection of DNA damage and repair. Methods Mol Biol. 1999; 113: 203-12[DOI][PubMed]
  • 14. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1): 184-91[PubMed]
  • 15. Jalali M, Hatami A, Kalantari H, Kalantar E. Mutagenicity Assessment of Two Herbal Medicines, Urtan and Carmint in Human Leukocytes by Single Cell Gel Electrophoresis. Saudi Pharm J. 2006; 14(2): 129
  • 16. Junqueira APF, Perazzo FF, Souza GHB, Maistro EL. Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo ma mmalian cell system. Genet Mol Biol. 2007; 30(3): 656-63
  • 17. Kalantari H, Jalali MT, Moein E. In Vitro Evaluation of Mutagenic Effect of Vitagnus and Shirafza in Human Leukocytes by Single Cell Gel Electrophoresis. Jundushapur J Nat Pharm Prod. 2007; 1(1): 26-31
  • 18. Hartmann A, Speit G. The contribution of cytotoxicity to DNA-effects in the single cell gel test (comet assay). Toxicol let. 1997; 90(2): 183-8
  • 19. Kalantari H, Panahi M, Izadinajafabadi A, Mohammadi E. Genotoxicity Determinations of Coriander Drop and Extract of Coriander Sativum in Cultured Fibroblast of Rat Embryo by Comet Assay. Saudi Pharm J . 2008; 16(1): 85
  • 20. Morkûnas V. Investigation of the genetic toxicology of dill essential oil and benzo (a) pyrene in mouse bone marrow by micronucleus test. Bioloija. 2002; 4: 14-6
  • 21. Hosseinzadeh H, Karimi GR, Ameri M. Effects of Anethum graveolens L. seed extracts on experimental gastric irritation models in mice. BMC Pharmacol. 2002; 2: 21[PubMed]
  • 22. Thukham-Mee W, Wattanathorn J. Evaluation of Safety and Protective Effect of Combined Extract of Cissampelos pareira and Anethum graveolens (PM52) against Age-Related Cognitive Impairment. Evid Based Complement Alternat Med. 2012; 2012: 674101[DOI][PubMed]
  • 23. Lazutka JR. Genotoxicity of dill (Anethum graveolensL.), peppermint (Mentha× piperita L.) and pine (Pinus sylvestris L.) essential oils in human lymphocytes and Drosophila melanogaster. Food Chem Toxicol. 2001; 39(5): 485-92
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