Jundishapur Journal of Natural Pharmaceutical Products

Published by: Kowsar

Determination of the Mutagenicity Potential of Dillsun Herbal Medicine by Single Cell Gel Electrophoresis in Rat Hepatocytes

Heibatullah Kalantari 1 , Mohsen Rezaei 1 , Maryam Salehcheh 1 , Mehrnoosh Moosavi 1 and Golnaz Varnaseri 1 , *
Authors Information
1 Department of Pharmacology and Toxicology, Medicinal Plants and Natural Products Research Center, School of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Natural Pharmaceutical Products: June 10, 2013, 8 (2); 55-59
  • Published Online: May 4, 2013
  • Article Type: Research Article
  • Received: December 1, 2012
  • Revised: January 21, 2013
  • Accepted: February 4, 2013
  • DOI: 10.17795/jjnpp-9156

To Cite: Kalantari H, Rezaei M, Salehcheh M, Moosavi M, Varnaseri G. Determination of the Mutagenicity Potential of Dillsun Herbal Medicine by Single Cell Gel Electrophoresis in Rat Hepatocytes, Jundishapur J Nat Pharm Prod. 2013 ; 8(2):55-59. doi: 10.17795/jjnpp-9156.

Copyright © 2013, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Kunle OF, Egharevba HO, Ahmadu PO. Standardization of herbal medicines-A review. Int J Biodivers Conserv. 2012; 4(3): 101-12
  • 2. Van Wyk BE, van Oudtshoorn B, Gericke N. Medicinal Plants of South Africa. 1997;
  • 3. McGaw LJ, Jager AK, van Staden J. Antibacterial, anthelmintic and anti-amoebic activity in South African medicinal plants. J Ethnopharmacol. 2000; 72(1-2): 247-63[PubMed]
  • 4. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001; 109 Suppl 1: 69-75[PubMed]
  • 5. Schimmer O, Hafele F, Kruger A. The mutagenic potencies of plant extracts containing quercetin in Salmonella typhimurium TA98 and TA100. Mutat Res. 1988; 206(2): 201-8[PubMed]
  • 6. van der Lelie D, Regniers L, Borremans B, Provoost A, Verschaeve L. The VITOTOX test, an SOS bioluminescence Salmonella typhimurium test to measure genotoxicity kinetics. Mutat Res. 1997; 389(2-3): 279-90[PubMed]
  • 7. Garaj-Vrhovac V, Zeljezic D. Comet assay in the assessment of the human genome damage induced by γ-radiation in vitro. Radiol Oncol. 2004; 38(1): 43-7
  • 8. Wong VWC, Szeto YT, Collins AR, Benzie IFF. THE COMET ASSAY: a biomonitoring tool for nutraceutical research. Curr Top in Nutr Res. 2005; 3(1): 1-14
  • 9. Dillsun Oral Drop.
  • 10. Vian L, Yusuf A, Guyomard C, Cano JP. The Liverbeads as a tool for the comet assay. Mutat Res. 2002; 519(1-2): 163-70[PubMed]
  • 11. Rubinstein LV, Shoemaker RH, Paull KD, Simon RM, Tosini S, Skehan P, et al. Comparison of in vitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines. J Natl Cancer Inst. 1990; 82(13): 1113-8[PubMed]
  • 12. Moneef MA, Sherwood BT, Bowman KJ, Kockelbergh RC, Symonds RP, Steward WP, et al. Measurements using the alkaline comet assay predict bladder cancer cell radiosensitivity. Br J Cancer. 2003; 89(12): 2271-6[DOI][PubMed]
  • 13. Speit G, Hartmann A. The comet assay (single-cell gel test). A sensitive genotoxicity test for the detection of DNA damage and repair. Methods Mol Biol. 1999; 113: 203-12[DOI][PubMed]
  • 14. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1): 184-91[PubMed]
  • 15. Jalali M, Hatami A, Kalantari H, Kalantar E. Mutagenicity Assessment of Two Herbal Medicines, Urtan and Carmint in Human Leukocytes by Single Cell Gel Electrophoresis. Saudi Pharm J. 2006; 14(2): 129
  • 16. Junqueira APF, Perazzo FF, Souza GHB, Maistro EL. Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo ma mmalian cell system. Genet Mol Biol. 2007; 30(3): 656-63
  • 17. Kalantari H, Jalali MT, Moein E. In Vitro Evaluation of Mutagenic Effect of Vitagnus and Shirafza in Human Leukocytes by Single Cell Gel Electrophoresis. Jundushapur J Nat Pharm Prod. 2007; 1(1): 26-31
  • 18. Hartmann A, Speit G. The contribution of cytotoxicity to DNA-effects in the single cell gel test (comet assay). Toxicol let. 1997; 90(2): 183-8
  • 19. Kalantari H, Panahi M, Izadinajafabadi A, Mohammadi E. Genotoxicity Determinations of Coriander Drop and Extract of Coriander Sativum in Cultured Fibroblast of Rat Embryo by Comet Assay. Saudi Pharm J . 2008; 16(1): 85
  • 20. Morkûnas V. Investigation of the genetic toxicology of dill essential oil and benzo (a) pyrene in mouse bone marrow by micronucleus test. Bioloija. 2002; 4: 14-6
  • 21. Hosseinzadeh H, Karimi GR, Ameri M. Effects of Anethum graveolens L. seed extracts on experimental gastric irritation models in mice. BMC Pharmacol. 2002; 2: 21[PubMed]
  • 22. Thukham-Mee W, Wattanathorn J. Evaluation of Safety and Protective Effect of Combined Extract of Cissampelos pareira and Anethum graveolens (PM52) against Age-Related Cognitive Impairment. Evid Based Complement Alternat Med. 2012; 2012: 674101[DOI][PubMed]
  • 23. Lazutka JR. Genotoxicity of dill (Anethum graveolensL.), peppermint (Mentha× piperita L.) and pine (Pinus sylvestris L.) essential oils in human lymphocytes and Drosophila melanogaster. Food Chem Toxicol. 2001; 39(5): 485-92
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments