Jundishapur Journal of Natural Pharmaceutical Products

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Determination of Cell Death Induced by Lovastatin on Human Colon Cell Line HT29 Using the Comet Assay

Marzieh Jafari 1 , Mohsen Rezaei 1 , * , Heibatullah Kalantari 1 and Mahmoud Hashemitabar 2
Authors Information
1 Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishpur University of Medical Sciences, Ahvaz, IR Iran
2 Cellular and Molecular Research Center, Ahvaz Jundishpur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Natural Pharmaceutical Products: November 2, 2013, 8 (4); 187-191
  • Published Online: November 2, 2013
  • Article Type: Research Article
  • Received: February 25, 2013
  • Revised: August 12, 2013
  • Accepted: August 31, 2013
  • DOI: 10.17795/jjnpp-10951

To Cite: Jafari M, Rezaei M, Kalantari H, Hashemitabar M. Determination of Cell Death Induced by Lovastatin on Human Colon Cell Line HT29 Using the Comet Assay, Jundishapur J Nat Pharm Prod. 2013 ; 8(4):187-191. doi: 10.17795/jjnpp-10951.

Copyright © 2013, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Van Cruchten S, Van Den Broeck W. Morphological and biochemical aspects of apoptosis, oncosis and necrosis. Anat Histol Embryol. 2002; 31(4): 214-23[DOI][PubMed]
  • 2. Bjelaković G, Nagorni A, Bjelaković M, Stamenković I, Arsić R, Katić V. Apoptosis: programmed cell death and its clinical implications. Med Biol. 2005; 12: 6-11
  • 3. Scovassi AI. Apoptosis and cell death. Apoptosis in the Retina. 2006; : 1-11
  • 4. Brown JM, Attardi LD. The role of apoptosis in cancer development and treatment response. Nat Rev Cancer. 2005; 5(3): 231-7[DOI][PubMed]
  • 5. Gomez-Lechon MJ, Ponsoda X, O'Connor E, Donato T, Castell JV, Jover R. Diclofenac induces apoptosis in hepatocytes by alteration of mitochondrial function and generation of ROS. Biochem Pharmacol. 2003; 66(11): 2155-67[PubMed]
  • 6. Bellosta S, Ferri N, Bernini F, Paoletti R, Corsini A. Non-lipid-related effects of statins. Ann Med. 2000; 32(3): 164-76[DOI][PubMed]
  • 7. Palinski W. Immunomodulation: a new role for statins? Nat Med. 2000; 6(12): 1311-2[DOI][PubMed]
  • 8. Garcia MJ, Reinoso RF, Sanchez Navarro A, Prous JR. Clinical pharmacokinetics of statins. Methods Find Exp Clin Pharmacol. 2003; 25(6): 457-81[PubMed]
  • 9. Di Matola T, D'Ascoli F, Luongo C, Bifulco M, Rossi G, Fenzi G, et al. Lovastatin-induced apoptosis in thyroid cells: involvement of cytochrome c and lamin B. Eur J Endocrinol. 2001; 145(5): 645-50[PubMed]
  • 10. Florent M, Godard T, Ballet JJ, Gauduchon P, Sola B. Detection by the comet assay of apoptosis induced in lymphoid cell lines after growth factor deprivation. Cell Biol Toxicol. 1999; 15(3): 185-92[PubMed]
  • 11. Rosl F. A simple and rapid method for detection of apoptosis in human cells. Nucleic Acids Res. 1992; 20(19): 5243[PubMed]
  • 12. Yasuhara S, Zhu Y, Matsui T, Tipirneni N, Yasuhara Y, Kaneki M, et al. Comparison of comet assay, electron microscopy, and flow cytometry for detection of apoptosis. J Histochem Cytochem. 2003; 51(7): 873-85[PubMed]
  • 13. Kumaravel TS, Vilhar B, Faux SP, Jha AN. Comet Assay measurements: a perspective. Cell Biol Toxicol. 2009; 25(1): 53-64[DOI][PubMed]
  • 14. Dhawan A, Bajpayee M, Parmar D. Comet assay: a reliable tool for the assessment of DNA damage in different models. Cell Biol Toxicol. 2009; 25(1): 5-32[DOI][PubMed]
  • 15. Collins AR, Dusinska M. Oxidation of cellular DNA measured with the comet assay. Methods Mol Biol. 2002; 186: 147-59[DOI][PubMed]
  • 16. Ritter D, Knebel J. Genotoxicity testing in vitro - development of a higher throughput analysis method based on the comet assay. Toxicol In Vitro. 2009; 23(8): 1570-5[DOI][PubMed]
  • 17. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35(3): 206-21[PubMed]
  • 18. Jiang Z, Zheng X, Lytle RA, Higashikubo R, Rich KM. Lovastatin-induced up-regulation of the BH3-only protein, Bim, and cell death in glioblastoma cells. J Neurochem. 2004; 89(1): 168-78[DOI][PubMed]
  • 19. Wong WW, Clendening JW, Martirosyan A, Boutros PC, Bros C, Khosravi F, et al. Determinants of sensitivity to lovastatin-induced apoptosis in multiple myeloma. Mol Cancer Ther. 2007; 6(6): 1886-97[DOI][PubMed]
  • 20. Agarwal B, Bhendwal S, Halmos B, Moss SF, Ramey WG, Holt PR. Lovastatin augments apoptosis induced by chemotherapeutic agents in colon cancer cells. Clin Cancer Res. 1999; 5(8): 2223-9[PubMed]
  • 21. Dimitroulakos J, Nohynek D, Backway KL, Hedley DW, Yeger H, Freedman MH, et al. Increased sensitivity of acute myeloid leukemias to lovastatin-induced apoptosis: A potential therapeutic approach. Blood. 1999; 93(4): 1308-18[PubMed]
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