Jundishapur Journal of Natural Pharmaceutical Products

Published by: Kowsar

Protective Effects of Crocin on Ischemia-reperfusion Induced Oxidative Stress in Comparison With Vitamin E in Isolated Rat Hearts

Mahin Dianat 1 , Mahdi Esmaeilizadeh 2 , * , Mohammad Badavi 1 , Ali Reza Samarbaf-zadeh 3 and Bahareh Naghizadeh 4
Authors Information
1 Department of Physiology, Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2 Department of Physiology, Diabetic Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
3 Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
4 Department of Pharmacology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Natural Pharmaceutical Products: May 3, 2014, 9 (2); e17187
  • Published Online: April 6, 2014
  • Article Type: Research Article
  • Received: December 27, 2013
  • Revised: January 21, 2014
  • Accepted: February 10, 2014
  • DOI: 10.17795/jjnpp-17187

To Cite: Dianat M, Esmaeilizadeh M, Badavi M, Samarbaf-zadeh A R, Naghizadeh B. Protective Effects of Crocin on Ischemia-reperfusion Induced Oxidative Stress in Comparison With Vitamin E in Isolated Rat Hearts, Jundishapur J Nat Pharm Prod. 2014 ; 9(2):e17187. doi: 10.17795/jjnpp-17187.

Copyright © 2014, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Dhalla NS, Elmoselhi AB, Hata T, Makino N. Status of myocardial antioxidants in ischemia-reperfusion injury. Cardiovasc Res. 2000; 47(3): 446-56[PubMed]
  • 2. Kinugasa Y, Ogino K, Furuse Y, Shiomi T, Tsutsui H, Yamamoto T, et al. Allopurinol improves cardiac dysfunction after ischemia-reperfusion via reduction of oxidative stress in isolated perfused rat hearts. Circ J. 2003; 67(9): 781-7[PubMed]
  • 3. Canbaz S, Duran E, Ege T, Sunar H, Cikirikcioglu M, Acipayam M. The effects of intracoronary administration of vitamin E on myocardial ischemia-reperfusion injury during coronary artery surgery. Thorac Cardiovasc Surg. 2003; 51(2): 57-61[DOI][PubMed]
  • 4. Jahanbakhsh Z, Rasoulian B, Jafari M, Shekarforoush S, Esmailidehaj M, Mohammadi MT, et al. Protective effect of crocin against reperfusion-induced cardiac arrhythmias in anaesthetized rats. EXCLI J. 2012; 11: 20-9
  • 5. Rajadurai M, Stanely Mainzen Prince P. Preventive effect of naringin on lipids, lipoproteins and lipid metabolic enzymes in isoproterenol-induced myocardial infarction in Wistar rats. J Biochem Mol Toxicol. 2006; 20(4): 191-7[DOI][PubMed]
  • 6. Ulrich-Merzenich G, Zeitler H, Vetter H, Kraft K. Synergy research: vitamins and secondary plant components in the maintenance of the redox-homeostasis and in cell signaling. Phytomedicine. 2009; 16(1): 2-16[DOI][PubMed]
  • 7. Montagna G, Hofer CG, Torres AM. Impairment of cellular redox status and membrane protein activities in kidneys from rats with ischemic acute renal failure. Biochim Biophys Acta. 1998; 1407(2): 99-108[PubMed]
  • 8. Upaganlawar A, Balaraman R. Effect of vitamin E and green tea on hemodynamic, electrocardiographic and some biochemical alterations in experimentally induced myocardial infarction in rats. Eur J Integr Med. 2010; 2(3): 135-41[DOI]
  • 9. Shen XC, Qian ZY. Effects of crocetin on antioxidant enzymatic activities in cardiac hypertrophy induced by norepinephrine in rats. Pharmazie. 2006; 61(4): 348-52[PubMed]
  • 10. Fernández JA. Advances in Phytomedicine. 2006; : 313-30[DOI]
  • 11. Assimopoulou AN, Sinakos Z, Papageorgiou VP. Radical scavenging activity of Crocus sativus L. extract and its bioactive constituents. Phytother Res. 2005; 19(11): 997-1000[DOI][PubMed]
  • 12. Soeda S, Ochiai T, Shimeno H, Saito H, Abe K, Tanaka H, et al. Pharmacological activities of crocin in saffron. J Nat Med. 2007; 61(2): 102-11[DOI]
  • 13. Hosseinzadeh H, Ziaei T. Effects of Crocus sativus stigma extract and its constituents, crocin and safranal, on intact memory and scopolamine-induced learning deficits in rats performing the Morris water maze task. J Med Plant. 2006; 5: 40-8
  • 14. Ochiai T, Shimeno H, Mishima K, Iwasaki K, Fujiwara M, Tanaka H, et al. Protective effects of carotenoids from saffron on neuronal injury in vitro and in vivo. Biochim Biophys Acta. 2007; 1770(4): 578-84[DOI][PubMed]
  • 15. Hosseinzadeh H, Sadeghnia HR, Ziaee T, Danaee A. Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia-reperfusion-induced oxidative damage in rats. J Pharm Pharm Sci. 2005; 8(3): 387-93[PubMed]
  • 16. Hosseinzadeh H, Modaghegh MH, Saffari Z. Crocus sativus L. (Saffron) extract and its active constituents (crocin and safranal) on ischemia-reperfusion in rat skeletal muscle. Evid Based Complement Alternat Med. 2009; 6(3): 343-50[DOI][PubMed]
  • 17. Cantoni O, Boscoboinik D, Fiorani M, Stauble B, Azzi A. The phosphorylation state of MAP-kinases modulates the cytotoxic response of smooth muscle cells to hydrogen peroxide. FEBS Lett. 1996; 389(3): 285-8[PubMed]
  • 18. Freedman JE, Keaney JF, Jr. Vitamin E inhibition of platelet aggregation is independent of antioxidant activity. J Nutr. 2001; 131(2): 374S-7S[PubMed]
  • 19. Griffin ME, Marcucci MJ, Cline GW, Bell K, Barucci N, Lee D, et al. Free fatty acid-induced insulin resistance is associated with activation of protein kinase C theta and alterations in the insulin signaling cascade. Diabetes. 1999; 48(6): 1270-4[PubMed]
  • 20. Zhou B, Wu LM, Yang L, Liu ZL. Evidence for alpha-tocopherol regeneration reaction of green tea polyphenols in SDS micelles. Free Radic Biol Med. 2005; 38(1): 78-84[DOI][PubMed]
  • 21. Goyal SN, Arora S, Sharma AK, Joshi S, Ray R, Bhatia J, et al. Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological and ultrastuctural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomedicine. 2010; 17(3-4): 227-32[DOI][PubMed]
  • 22. Garcia-Villalon AL, Monge L, Fernandez N, Salcedo A, Narvaez-Sanchez R, Dieguez G. Coronary response to diadenosine pentaphosphate after ischaemia-reperfusion in the isolated rat heart. Cardiovasc Res. 2009; 81(2): 336-43[DOI][PubMed]
  • 23. Son IS, Kim JH, Sohn HY, Son KH, Kim JS, Kwon CS. Antioxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam (Dioscorea spp.), on high-cholesterol fed rats. Biosci Biotechnol Biochem. 2007; 71(12): 3063-71[PubMed]
  • 24. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2): 351-8[DOI]
  • 25. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47(3): 469-74[PubMed]
  • 26. Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105: 121-6[PubMed]
  • 27. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical role as a component of glutathione peroxidase. Science. 1973; 179(4073): 588-90[PubMed]
  • 28. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1): 70-6[DOI][PubMed]
  • 29. Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol. 1999; 299: 15-27[PubMed]
  • 30. Suchalatha S, Shyamala Devi CS. Protective effect of Terminalia chebula against experimental myocardial injury induced by isoproterenol. Indian J Exp Biol. 2004; 42(2): 174-8[PubMed]
  • 31. Rhoden E, Teloken C, Lucas M, Rhoden C, Mauri M, Zettler C, et al. Protective effect of allopurinol in the renal ischemia--reperfusion in uninephrectomized rats. Gen Pharmacol. 2000; 35(4): 189-93[PubMed]
  • 32. Abdollahi M, Ranjbar R, Shadnia S, Nikfar S, Rezaie A. Pesticides and oxidative stress: a review. Med Sci Monit. 2004; 10: 141-7
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments