STUDY OF THE PROTECTIVE EFFECT OF RAPHANUS SATIVUS (RADISH) SEED IN LIVER TOXICITY INDUCED BY CARBON TETRACHLORIDE IN MICE
Jundishapur Journal of Natural Pharmaceutical Products: 4 (1); 24-31 Article Type: Research Article
December 13, 2009
January 3, 2010
M. STUDY OF THE PROTECTIVE EFFECT OF RAPHANUS SATIVUS (RADISH) SEED IN LIVER TOXICITY INDUCED BY CARBON TETRACHLORIDE IN MICE,
Jundishapur J Nat Pharm Prod.
Online ahead of Print
It is well known that some herbal medicine play an important role in therapy. The hydro-alcoholic extract of Radish seed was obtained by maceration technique. Animals were weighed and divided in eight groups (each group consists of seven mice). The control groups received normal saline and olive oil for four consecutive days. The positive control group received normal saline in days first and 4th and CCL
4 (0.2ml/kg) in days 2nd and 3rd. The test groups received oral crude extracts in doses of (100, 200, 400, 600, 800 mg/kg) on day first and day fourth. Then on day second and 3rd they received oral CCL 4 one hour before crude extract administration. On day fifth all groups were weighed and then administered intrapretoneal (ip) with hexobarbital sodium (0.25mg/kg) to determine the sleeping time. Blood samples was withdrawn and serum was prepared for AST and ALT activities. Liver was removed and kept in 10% formalin solution for histopathological studies. Results of histopathological findings showed that groups received (600 and 800 mg/kg) were significant (reduction in liver damage) as compared with positive control group. Also, results of serum enzyme activities were analyzed by one-way ANOVA method indicated that these two groups (600 and 800 mg/kg) were also significant as compared with CCL 4 group (p<0.01). In conclusion according to results obtained from crude extract of Radish seed in dose of (600 and 800 mg/kg) may be good enough to protect liver damage induced by CCL 4.
Liver, Radish seed, Carbon tetrachloride, AST, ALT enzymes, Mice.
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