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First Survey of Metallo-β–Lactamase Producers in Clinical Isolates of Pseudomonas aeruginosa From a Referral Burn Center in Kurdistan Province


Enayatollah kalantar 1 , * , Vahideh Torabi 2 , Heiman Salimizand 3 , Fariborz Soheili 4 , Soheila Beiranvand 5 , Mohammad Mehdi Soltan Dallal 6


1 Envirronmental Health Resaerch Center, Kurdistan University of Medical Sciences, Sanandaj, IR Iran

2 Department of Biological Sciences, Islamic Azad University, Sciences Research Branch, Tehran, IR Iran

3 Department of Microbiology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran

4 School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran

5 Qods Hospital, Kurdistan University of Medical Sciences, Sanandaj, IR Iran

6 Division of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran


Jundishapur Journal of Natural Pharmaceutical Products: 7 (1); e94148
Published Online: January 04, 2012
Article Type: Research Article
Received: May 20, 2019
Accepted: December 05, 2011




Background: Treatment of infectious diseases is becoming more challenging with each passing year. This is especially true for infections caused by Pseudomonas aeruginosa, an opportunistic pathogen with the ability to rapidly develop resistance to multiple classes of antibiotics.

Objectives: This study was conducted to determine the prevalence of metallo-β-lactamase (MBL)–producing strains among multidrug-resistant P. aeruginosa strains isolated from burn patients.

Materials and Methods: The isolates were identified, tested for susceptibility to various antimicrobial agents, and screened for the presence of MβLs by using the double-disk synergy test. The minimal inhibitory concentration of imipenem was determined by microplate broth dilution method on Mueller-Hinton agar. To detect VIM, SIM, and GIM MBLs, the isolates were subjected to polymerase chain reaction.

Results: In this study, we identified 100 P. aeruginosa isolates from 176 clinical specimens obtained from burn patients. The isolates showed maximum resistance to ampicillin (100%), ceftazidime (94%), and ceftriaxone (89%). The CLSI-MBL phenotypic test showed that of the 100 P. aeruginosa isolates, 22 (22%) were positive for MBL production in the double-disk synergy test. Of the 22 MBL-positive P. aeruginosa isolates, 8 were resistant to imipenem. PCR analysis showed that 8 isolates were positive for blaVIM1. The other genes blaSIM1 and blaGIM1 were not detected.

Conclusions: The study results demonstrate the serious therapeutic threat of the spread of MBL producers among P. aeruginosa populations. Metallo-β-lactamases were detected in 22% of imipenem-resistant P. aeruginosa isolates. Early detection and infection-control practices are the best antimicrobial strategies for this organism; therefore, systematic surveillance to detect MBL producers is necessary.


Burn Patients P. aeruginosa Metallo-β-lactamase Imipenem Resistance

© 2012, Jundishapur Journal of Natural Pharmaceutical Products. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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