Effects of Hydroalcoholic Extract of Hogweed and Anise on Broiler Meat Quality, Immune Responses, and Intestinal Microflora and Morphology

AUTHORS

Majid Hemati 1 , Jafar Fakhraei 1 , * , Akbar Yaghobfar 2 , Hossein Mansoori Yarahmadi 1

1 College of Agriculture and Natural Resource, Arak Branch, Islamic Azad University, Arak, Iran

2 Animal Science Research Institute, Agricultural Research Education and Extension Organization, Karaj, Iran

How to Cite: Hemati M, Fakhraei J , Yaghobfar A, Mansoori Yarahmadi H. Effects of Hydroalcoholic Extract of Hogweed and Anise on Broiler Meat Quality, Immune Responses, and Intestinal Microflora and Morphology, Jundishapur J Nat Pharm Prod. Online ahead of Print ; In Press(In Press):e90870. doi: 10.5812/jjnpp.90870.

ARTICLE INFORMATION

Jundishapur Journal of Natural Pharmaceutical Products: In Press (In Press); e90870
Published Online: September 25, 2019
Article Type: Research Article
Received: February 20, 2019
Revised: May 13, 2019
Accepted: May 19, 2019
Uncorrected Proof scheduled for 15 (1)
Crossmark

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background: Poultry meat has high contents of polyunsaturated fatty acids that can cause off-flavors, off-odors, and reduce meat quality and shelf life. Replacement of phytogenic feed additives (PFAs) with chemical additives are highly considered, which reduces the potential risk of using poultry meat.

Objectives: The current study aimed at determining the effects of hydroalcoholic extract of hogweed (Heracleum persicum) and anise (Pimpinella anisum L.) on broiler meat quality, immune responses, and intestinal microflora and morphology.

Methods: The current study was conducted on 400 male Ross 308 chickens divided into five treatment groups, based on a completely randomized design with four replicates of 20 broilers. The control group was not fed any feed additives, whereas the other groups received 100 mg/kg of probiotics (Primalac®), 200 mg/kg of hogweed extract, 200 mg/kg of anise extract, and 200 mg/kg of oxytetracycline, respectively. On day 35 of chicken breeding, the immune responses were investigated. At the end of chicken breeding, meat quality was analyzed. Also, the jejunal and ileal contents were investigated in terms of intestinal morphology and microflora.

Results: The findings indicated that the highest immune response belonged to the anise extract treatment. The best meat quality belonged to treatments with hogweed and anise extracts (P < 0.05). Also, the lowest number of harmful intestinal bacteria belonged to hogweed extract treatment (P < 0.05). Morphological characteristics of the intestine improved under the effects of hogweed and anise extract treatments (P < 0.05).

Conclusions: Therefore, inclusion of 200 mg/kg of hogweed and anise extracts in broiler diet is suggested to improve meat quality, immune responses, and intestinal morphology as well as reduce the number of harmful bacteria in the ileum.

Keywords

Hydroalcoholic Extract Heracleum persicum Pimpinella anisum Broiler

Copyright © 2019, Jundishapur Journal of Natural Pharmaceutical Products. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

1. Background

Long-chain unsaturated fatty acids are beneficial for human health. Poultry meat has high contents of polyunsaturated fatty acids. However, increasing the amount of unsaturated fatty acids increases the sensitivity of chicken fat to oxidative degradation, which changes the taste and nutritional composition of the meat. Consumer demand to reduce synthetic preservatives increased the interest in the antioxidant and antimicrobial properties of medicinal herb extracts (1, 2). Free radicals are one of the most important factors in the oxidation of food, which cause oxidation of fats and oils. In addition, byproducts of oxidation of lipids can have negative effects on the food components. As a result, degradation of vitamins and essential fatty acids and production of toxic compounds in food products can lead to undesirable consequences such as inflammatory diseases, cancer, and immunodeficiency in the human body (3). Therefore, the use of antioxidants in order to slowdown the oxidation process in foodstuff and extend the lifetime of opened food products seems necessary. The replacement of phytogenic feed additives (PFAs) with chemical preservatives raised much attention it reduces the adverse effects caused by the consumption of chemical preservatives. Medicinal plants are economic supplements that can be used in poultry farming and their antioxidant effects are evident in the phenolic compounds (3, 4). Heracleum persicum and Pimpinella anisum L. are two medicinal plants from the Apiaceae family with numerous applications in food and drug industries (5). H. persicum, commonly known as Persian hogweed, is recognized as a native flowering plant in Iran (6). There are generally 10 hogweed species, four of which are endemic to Iran (7). The main constituents of hogweed seed include trans-anetole, hexyl butyrate, octyl acetate, and hexyl isobutyrate (8). Research on hogweed suggests its beneficial effects on growth (9-11), immune function (12, 13), hematological parameters (14), and intestine morphometrics (9). Studies also show that addition of hogweed to diet can prevent oxidative changes by free radicals and other reactive species through radical scavenging and lipid peroxidation inhibition (15). On the other hand, anise is described as a potential natural growth promotor for poultry (16), which can be used as an antibiotic alternative. Anise seeds contain different constituents including trans-anetole, eugenol, anisaldehyde, polyacetylenes, methyl chavicol, scopoletin, estragole, coumarins, umbelliferone, terpene hydrocarbons, estrols, and polyenes (8). In addition, some of the positive effects of anise seeds on animals’ performance are attributed to their favorable effects on growth (17-19), antioxidant status (20), ileal nutrient digestibility (21), anti-inflammatory responses, and immune system (22). Also, Hong et al., (23) showed that in chickens fed with anise, the breast muscles had more tenderness in comparison with the antibiotic and control groups.

2. Objectives

The present study aimed at evaluating the effects of the inclusion of hydroalcoholic extract of hogweed or anise in diet on broiler meat quality, immune responses, and intestinal microflora and morphology.

3. Methods

3.1. Preparation of Extracts and Diets

The hogweed and anise seed extracts were purchased from a reputable corporation (Know Tech. Pharmaceutical Co. Tehran, Iran). The entire content of each extract was measured using the Folin-Ciocalteu reagent (24). The hogweed and anise extracts contained 42.27% and 35.80% phenolic compounds, respectively. In total, 400 male Ross 308 chicks (42 ± 3.0 g; one day old) were supplied by Toyoorbarekat Company (Tehran, Iran). The chicks were assigned to five groups based on a completely randomized design in four replicates (20 chicks per replicate). During the experiments, the chickens had free access to water and food in mash form. To meet their nutritional needs, three isocaloric and isonitrogenous dietary formulations were used for the starter (1 - 10), grower (11 - 24), and finisher (25 - 42) periods, as recommended by National Research Council (Table 1). No feed additives were used in the control group, whereas the 2nd, 3rd, 4th, and 5th groups received 100 mg/kg of probiotics (PrimaLac® 454; Star-Labs Clarksdale, USA), 200 mg/kg of hogweed extract, 200 mg/kg of anise extract, and 200 mg/kg of oxytetracycline 20% (Fidax®; Darusazan Iran Co., Tehran, Iran), respectively. In the present study, PrimaLac® 454 feed grade, a multi-strain powder preparation (1 × 108 CFU/g), contained Lactobacillus casei, Bifidobacterium thermophilum, Enterococcus faecium, and Lactobacillus acidophilus (all at 2.5 × 107 CFU/g). This preparation was selected considering the variability and availability of bacterial strains such as Lactobacillus species (25).

Table 1. Composition and Analysis of Experimental Diets in Different Periods
Period
Starter (1 - 10 d)Grower (11 - 24 d)Finisher (25 - 42 d)
Ingredient (%)
Corn (8.5% CP)54.3258.0066.00
Soybean meal (44% CP)39.8035.2229.00
Soya oil2.153.302.00
Oyster shell0.80.700.65
Di-calcium phosphate1.751.651.30
Mineral premixa0.250.250.25
Vitamin premixa0.250.250.25
Salt0.230.230.20
DL-methionine0.280.200.20
Lysine0.170.200.15
Anise extractb (the 2nd diet)0.020.020.02
Hogweed extractc (the 3rd diet)0.020.020.02
Oxytetracycline antibioticd (the 4th diet)0.020.020.02
Probiotic primalac®e (the 5th diet)0.010.010.01
Chemical analysis
Metabolizable energy (kcal/kg)298031003150
Crude protein (%)23.0021.2419.08
Methionine (%)0.510.500.47
Methionine + cysteine (%)0.950.810.76
Lysine (%)1.291.231.04
Threonine (%)0.800.710.63
Tryptophan (%)0.350.260.22
Calcium (%)0.980.900.76
Available phosphorus (%)0.460.440.37
Sodium (%)0.200.200.20

aMinerals-vitamins premix provided the following per kilogram of diet: 99.2 mg manganese, 85 mg zinc, 50 mg iron, 10 mg copper, 0.2 mg selenium, 13 mg iodine, 44000 IU vitamin A, 7200 IU vitamin D3, 440 IU vitamin E, 40 mg vitamin K, 70 mg cobalamin, 65 mg thiamine, 320 mg riboflavin, 290 mg pantothenic acid, 1220 mg niacin, 65 mg pyridoxine, 22 mg biotin, and 270 mg choline chloride.

bAnise extract in the total period of production was added to control diet and tested as 2nd ration.

cHogweed extract in the total period of production was added to control diet and tested as 3rd ration.

dOxytetracycline (20%) in the total period of production was added to control diet and tested as 4th ration.

ePrimalac® probiotics in the total period of production were added to control diet and tested as 5th ration.

3.2. Meat Quality

On day 42 of the trial, three broiler chicks were selected per replicate according to their average body weight after 12 hours of fasting. The animals were sacrificed through cervical dislocation, followed by bleeding. After removing the breast muscles, they were stored separately in plastic bags to examine meat quality (e.g., malondialdehid (MDA) content, pH, and water holding capacity (WHC) at 0, 24, and 48 hours after slaughtering at 4°C. The breast muscle pH was measured at 2.5 cm below the surface with a pH meter model pH-211 (Hanna Instruments Inc., Italy) equipped with a spear electrode. Moreover, MDA content, recognized as an indicator of lipid oxidation, was measured in breast muscles using thiobarbituric acid method described by Witte et al. (26). Also, after placing 0.5 g breast meat samples in aluminum foils between metal plates, WHC was measured by leaving the sample under water for one minute at a pressure of 130 bar (27).

3.3. Immune Responses

In each replicate, two chicks were randomly selected on day 28 of age and received peritoneal injections of 7% sheep red blood cell (SRBC) suspension (0.5 mL). Seven days following the SRBC challenge, blood samples were collected. According to the microhemagglutination technique suggested by Qureshi and Havanstein (28), the level of anti-SRBC antibody was measured. The antibody data are presented as log2 of the reciprocal of the highest dilution yielding visible agglutination.

3.4. Intestinal Morphometry

At the end of experiment (42 days), two chicks were randomly sacrificed per replicate, and the intestinal middle segments (jejunum) were separated. Almost 1.5 cm of the intestine was sampled and positioned for 24 hours after washing with 85% saline; then, the samples were placed in formalin 10%. After preparation of the samples, a light microscope (Olympus Co. Tokyo, Japan) equipped with a computer was used to measure the villus width, villus height (from the villus tip to the villus-crypt junction), villus height to crypt depth ratio (VH/CD), and crypt depth (invagination depth between the adjacent villi), according to a method described by Sakamoto et al. (29). The villus height and width were determined to calculate the villus surface area:

2π×(W2)×L

Where, W represents the villus width and L denotes the villus height.

3.5. Ileal Bacterial Populations

On day 42 of the trial, to investigate the ileal bacterial populations, 1 g of ileal content of two randomly selected chicks per replicate was added to a sterile physiological salt solution (9 mL), and bacterial populations were examined based on the method described by Mookiah et al. (30). Bacterial populations are expressed as log10 CFU/g of ileal content. It should be noted that all bacterial enumerations were carried out in duplicate.

3.6. Statistical Analysis

For statistical analysis, the general linear model was applied in SAS (31). A randomized design was applied in ANOVA. Duncan’s multiple-range test was performed to measure the mean values (32). P < 0.05 was considered the level of significant.

4. Results

The results related to meat quality (pH, MDA content, and WHC) of breast muscles are presented in figures 1, 2, and 3. After meat collection, the results indicated that pH was not majorly influenced by treatments (P > 0.05), whereas the MDA content (24 and 48 hours) was significantly lower in groups fed with PFAs, compared to the other groups (P < 0.05). Also, supplementation of diet with 200 mg/kg hogweed and anise extracts increased the meat WHC (P < 0.05).

Effects of the inclusion of PrimaLac<sup>®</sup> probiotics, oxytetracycline, and hogweed and anise extracts in diet on meat ph at 0, 24, and 48 hours after slaughtering of broilers
Figure 1. Effects of the inclusion of PrimaLac® probiotics, oxytetracycline, and hogweed and anise extracts in diet on meat ph at 0, 24, and 48 hours after slaughtering of broilers
Effects of the inclusion of PrimaLac<sup>®</sup> probiotics, oxytetracycline, and hogweed and anise extracts in diet on meat MDA content (mg/g) at 0, 24, and 48 hours after slaughtering of broilers; different letters indicate significant differences among treatments (P &lt; 0.05).
Figure 2. Effects of the inclusion of PrimaLac® probiotics, oxytetracycline, and hogweed and anise extracts in diet on meat MDA content (mg/g) at 0, 24, and 48 hours after slaughtering of broilers; different letters indicate significant differences among treatments (P < 0.05).
Effects of the inclusion of PrimaLac<sup>®</sup> probiotics, oxytetracycline, hogweed and anise extracts in diet on meat WHC (%) at 0, 24, and 48 hours after slaughtering of broilers; different letters indicate significant differences among treatments (P &lt; 0.05).
Figure 3. Effects of the inclusion of PrimaLac® probiotics, oxytetracycline, hogweed and anise extracts in diet on meat WHC (%) at 0, 24, and 48 hours after slaughtering of broilers; different letters indicate significant differences among treatments (P < 0.05).

Figure 4 presents the effects of the supplementation of diet with feed additives on humoral immunity (anti-SRBC titer). Based on the findings, treatments did not affect the immune responses.

Effects of the Inclusion of PrimaLac<sup>®</sup> probiotics, oxytetracycline, and hogweed and anise extracts in diet on SRBC antibody titer (Log<sub>10</sub>) of broiler
Figure 4. Effects of the Inclusion of PrimaLac® probiotics, oxytetracycline, and hogweed and anise extracts in diet on SRBC antibody titer (Log10) of broiler

The effects of experimental treatments on the ileum microbial population and intestinal morphology are shown in Table 2. In comparison with the controls, a significantly lower ileal content of coliforms was observed in the treatment groups (P < 0.05). Also, ileal Escherichia coli and total aerobic bacteria counts were significantly lower in the hogweed extract group in comparison with the other groups (P < 0.05). Moreover, the results showed that treatments did not influence the crypt depth or villus width (P > 0.05). The treatment groups showed significantly higher villus height, VH/CD, and villus area in comparison with the controls (P < 0.05).

Table 2. Effects of the Inclusion of PrimaLac® Probiotics, Oxytetracycline, and Hogweed and Anise Extracts in Diet on Intestinal Microflora and Morphology of Broilers
TraitTreatmentStatistical Parameter
ControlPrimaLac® ProbioticsOxytetracyclineHogweed ExtractAnise ExtractSEMP Value
Intestinal microflora log10 (CFU/g)
Total of aerobic bacteria10.49a7.95b9.43a6.21c8.47a, b0.370.04
Escherichia coli3.53a3.43a3.73a1.87b3.63a0.140.03
Coliform bacteria6.52a4.68b2.22c2.16c4.79b0.540.01
Morphology
Villus height (µm)1046.00c1217.4a, b1134.6b1193.6a, b1224.2a26.850.001
Villus width (µm)120.20114.8112.60120.40107.205.090.347
Crypt depth (µm)119.00112.20112.60110.00122.405.600.532
VH/CDd8.79b10.85a10.08a10.85a10.00a0.3760.011
Villus area (mm2)0.455b0.495a, b0.460a, b0.500a0.477a, b0.0150.024

Abbreviation: SEM, standard error of mean.

aMeans within a row without common superscript are significantly different at P < 0.05.

bMeans within a row without common superscript are significantly different at P < 0.05.

cMeans within a row without common superscript are significantly different at P < 0.05.

dVillus height to crypt depth.

5. Discussion

In recent years, improvement of meat quality using plant extract supplementations is highlighted in poultry farming since consumers are concerned about poultry meat quality. Reduction of meat pH causes reduced WHC, and as a result the nutritional value of meat, which depends on the amount of protein denaturation in tissue, is eliminated. The current study did not find significant effects of treatment on meat pH, but WHC was higher than those of the medicinal herb extract groups in agreement with the result of Jiang et al. (33). The meat oxidative stability may be affected by feeding and production conditions of animals (33). The total phenol content of the breast meat of birds receiving diets containing supplements was significantly different and has higher antioxidant activities compared to those of controls (3). Supplementation of diet with 200 mg/kg hogweed or anise extract slightly decreases the MDA content of meat (P < 0.05). MDA, as the end product of lipid peroxidation, is an index for ROS (reactive oxygen species)-induced biological damage (34), isolated from urine, blood, and tissue. Therefore, PFA supplements are speculated to reduce lipid peroxidation and consequently increase meat quality. However, the effects of supplements with hogweed and anise extracts on meat lipid peroxidation and quality are not examined in broiler chickens. Accordingly, PFA supplements are potential additives to improve meat quality in broiler chickens. Amad et al. (35), reported that broilers fed phytogenics containing essential oil of star anise as lead active components had an improved feed-conversion ratio and such effects can result in higher economic efficiency in broiler meat production. The consequences of poultry production for the environment, food safety, and animal welfare issues are now part of consumers’ opinions and demands. Replacement strategies based on novel or alternative feeding practices without antibiotics are favored by the industry.

SRBC antibody titer was unaffected by either probiotic, antibiotic, or PFAs supplementation (Figure 4). Previous studies show that supplementation of diet with PFAs stimulates cellular and humoral immune functions (10, 11, 36), which might be attributed to its content in furanocoumarins and flavonoids. Amresh et al. (37), showed that flavonoids and polyphenolic compounds help the immune system through antioxidant activity. The improved immune function by prebiotics supplementation can be attributed to preferential colonization of beneficial bacteria and microbial products that produce immune response. On the contrary, Eftekhari et al. (9) observed that broiler diet supplemented with hogweed did not have any significant effects on primary and secondary antibody responses. Furthermore, Hong et al. (23), reported that total antibody titer was not affected by the commercial blend of phytogenics supplementation, which was similar to the current study findings regarding PFAs supplementation.

Based on the results of intestinal microflora and morphology analysis, administration of synthetic (PrimaLac® probiotics and oxytetracycline) or natural (hogweed and anise extracts) food additives improves the poultry health and productivity by modulation of intestinal microbial ecosystem. Inclusion of prebiotics (38) and PFAs (39) in diet enriched beneficial microbiota and decreased pathogenic bacteria colonization (e.g., E. coli). In a nutshell, medicinal plant extract supplements are economic alternatives, which can also be added to poultry diet in order to enrich intestinal microbiota.

In diets containing PFA, villus height, VH/CD, and villus area were notably higher in broiler chicks fed with synthetic (PrimaLac® probiotics and oxytetracycline) or natural (hogweed and anise) food additives, which promote the absorption of nutrients. The present study results were not in congruence with earlier reports, which indicated that supplementation of diet with plant extract increased the villus height (23) and VH/CD ratio (9, 11). Moreover, Amad et al. (21), showed that PFA supplementation causes jejunal morphological changes, which may affect the absorption of nutrients and improve feed conversion ratio (FCR). In another study by Viveros et al. (40), phenolic compounds could change the intestinal microflora and morphology and improve the intestinal bacteria biodiversity in broiler chicks. Also, a recent report by Mohiti-Asli and Ghanaatparast-Rashti (39) showed that oregano essential oil and phytogenic blend had similar antimicrobial activities, despite differences in their effects, on the intestinal morphological features. Therefore, adding phytogenics to broiler diet should be relied on the histomorphometry results of the small intestine rather than antimicrobial activities. In sum, it can be stated that PFA supplementation synergistically improves weight gain and FCR by modulating the intestinal system.

5.1. Conclusions

The present study results indicated that hogweed and anise extracts could be potential natural promoters of poultry growth and proper probiotic and antibiotic alternatives. Addition of PFAs had more significant effects on meat quality, compared to antibiotics and probiotics. Therefore, it is suggested to include 200 mg/kg PFAs in the diet of broiler chicks to improve the intestinal morphology and decrease harmful bacterial populations in the ileum. However, the results of different studies on supplementation of broiler diet with hydroalcoholic extract of hogweed and anise are still controversial, which could be due to the differences among various medicinal plants and their compositions as well as housing condition and health stage of examined animals. According to these variables, diets supplemented with 200 mg/kg extract of hogweed or anise were suitable to improve the broiler meat quality. Due to the fact that the report on supplementation with hogweed or anise and their active components in broilers is limited, the hogweed or anise and their active components could serve as a novel nutritional means of improving broiler meat quality, immune responses, and intestinal microflora and morphology. These effects can result in higher economic efficiency in broiler meat production. More detailed studies are still needed to elucidate the mode of action of hogweed and anise ingredients on nutritional and physiological response of broilers under various circumstances.

Footnotes

References

  • 1.

    Botsoglou NA, Christaki E, Fletouris DJ, Florou-Paneri P, Spais AB. The effect of dietary oregano essential oil on lipid oxidation in raw and cooked chicken during refrigerated storage. Meat Sci. 2002;62(2):259-65. doi: 10.1016/s0309-1740(01)00256-x. [PubMed: 22061420].

  • 2.

    Luna A, Labaque MC, Zygadlo JA, Marin RH. Effects of thymol and carvacrol feed supplementation on lipid oxidation in broiler meat. Poult Sci. 2010;89(2):366-70. doi: 10.3382/ps.2009-00130. [PubMed: 20075292].

  • 3.

    Jang A, Liu XD, Shin MH, Lee BD, Lee SK, Lee JH, et al. Antioxidative potential of raw breast meat from broiler chicks fed a dietary medicinal herb extract mix. Poult Sci. 2008;87(11):2382-9. doi: 10.3382/ps.2007-00506. [PubMed: 18931191].

  • 4.

    Gladine C, Morand C, Rock E, Bauchart D, Durand D. Plant extracts rich in polyphenols (PERP) are efficient antioxidants to prevent lipoperoxidation in plasma lipids from animals fed n−3 PUFA supplemented diets. Anim Feed Sci Tech. 2007;136(3-4):281-96. doi: 10.1016/j.anifeedsci.2006.09.003.

  • 5.

    Nazemi A, Hashemi M, Khataminejad MR, Pourshamsian K. [Antimicrobial activity of aqueous and methanol extracts of Heracoleum Persicum]. Med Sci J. 2005;15(2):91-4. Persian.

  • 6.

    Parsa A. Flore de L'Iran. Tehran, Iran: Imprimerie Danesh Press; 1951.

  • 7.

    Mozaffarian V. [A dictionary of iranian plant names]. Tehran, Iran: Farhang Moaser Publishers; 2007. Persian.

  • 8.

    Gulcin I, Oktay M, Kireccı E, Kufrevioglu OI. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chem. 2003;83(3):371-82. doi: 10.1016/s0308-8146(03)00098-0.

  • 9.

    Eftekhari SM, Hassanabadi A, Tahmasebi A, Golian A, Nassiri-Moghaddam H. The effect of hydroalcoholic extract of angelica (Heracleum persicum) fruit on performance, immune responses, small intestine histology, haematological parameters and carcass characteristics of broiler chickens. J Appl Anim Res. 2018;46(1):1336-43. doi: 10.1080/09712119.2018.1505621.

  • 10.

    Jamshidparvar A, Javandel F, Seidavi A, Pena Blanco F, Martinez Marin AL, Aviles Ramirez C, et al. Effects of golpar (Heracleum persicum Desf.) and probiotics in drinking water on performance, carcass characteristics, organ weights, blood plasma constituents, and immunity of broilers. Environ Sci Pollut Res Int. 2017;24(30):23571-7. doi: 10.1007/s11356-017-9983-4. [PubMed: 28852951].

  • 11.

    Mohiti-Asli M, Ghanaatparast-Rashti M. Comparison of the effect of two phytogenic compounds on growth performance and immune response of broilers. J Appl Anim Res. 2017;45(1):603-8. doi: 10.1080/09712119.2016.1243119.

  • 12.

    Asgarpanah EJ, Dadashzadeh GM, Ahmadi M, Ranjbar R, Ardebily MSA. Chemistry, pharmacology and medicinal properties of Heracleum persicum Desf. J Med Plants Res. 2012;6(10):1813-20. doi: 10.5897/jmpr11.1716.

  • 13.

    Hajhashemi V, Sajjadi SE, Heshmati M. Anti-inflammatory and analgesic properties of Heracleum persicum essential oil and hydroalcoholic extract in animal models. J Ethnopharmacol. 2009;124(3):475-80. doi: 10.1016/j.jep.2009.05.012. [PubMed: 19467316].

  • 14.

    Ciftci M, Guler T, Dalkilic B, Ertas N. Effect of Heracleum persicum extract on performance and some haematological parameters in broiler chicks. Res Opinion Anim Vet Sci. 2014;4(9):522-5.

  • 15.

    Coruh N, Celep AGS, Ozgokce F. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food Chem. 2007;100(3):1237-42. doi: 10.1016/j.foodchem.2005.12.006.

  • 16.

    Mehmet C, Talat G, Bestami D, Nihat EO. The effect of anise oil (Pimpinella anisum L.) on broiler performance. Int J Poult Sci. 2005;4(11):851-5. doi: 10.3923/ijps.2005.851.855.

  • 17.

    Charal JW, Bidner TD, Southern LL, Janes ME, Gutierrez ME, Lavergne TA. Anise oil dosage and its effect on growth performance and jejunal lesions during a Clostridium perfringens challenge in battery trials, and growth performance in a floor pen trial1,2. J Appl Poultr Res. 2017;26(2):240-52. doi: 10.3382/japr/pfw068.

  • 18.

    El-Deek AA, Attia YA, Hannfy MM. Effect of anise (Pimpinella anisum), ginger (Zingiber officinale roscoe) and fennel (Foeniculum vulgare) and their mixture on performance of broilers. Archiv fur Geflugelkunde. 2002;67(2):92-6.

  • 19.

    Reyer H, Zentek J, Manner K, Youssef IMI, Aumiller T, Weghuber J, et al. Possible molecular mechanisms by which an essential oil blend from star anise, rosemary, thyme, and oregano and saponins increase the performance and ileal protein digestibility of growing broilers. J Agric Food Chem. 2017;65(32):6821-30. doi: 10.1021/acs.jafc.7b01925. [PubMed: 28722406].

  • 20.

    Ding X, Yang CW, Yang ZB. Effects of star anise (Illicium verum Hook.f.), essential oil, and leavings on growth performance, serum, and liver antioxidant status of broiler chickens. J Appl Poultr Res. 2017;26(4):459-66. doi: 10.3382/japr/pfx014.

  • 21.

    Amad AA, Wendler KR, Zentek J. Effects of a phytogenic feed additive on growth performance, selected blood
    criteria and jejunal morphology in broiler chickens. Emirates J Food Agric. 2013;25(7):549. doi: 10.9755/ejfa.v25i7.12364.

  • 22.

    Mueller K, Blum NM, Mueller AS. Examination of the anti-inflammatory, antioxidant, and xenobiotic-inducing potential of broccoli extract and various essential oils during a mild DSS-Induced colitis in rats. ISRN Gastroenterol. 2013;2013:710856. doi: 10.1155/2013/710856. [PubMed: 23533793]. [PubMed Central: PMC3603549].

  • 23.

    Hong JC, Steiner T, Aufy A, Lien TF. Effects of supplemental essential oil on growth performance, lipid metabolites and immunity, intestinal characteristics, microbiota and carcass traits in broilers. Livestock Sci. 2012;144(3):253-62. doi: 10.1016/j.livsci.2011.12.008.

  • 24.

    Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc. 2007;2(4):875-7. doi: 10.1038/nprot.2007.102. [PubMed: 17446889].

  • 25.

    Kafilzadeh F, Payandeh S, Gomez-Cortes P, Ghadimi D, Schiavone A, Martínez Marín AL. Effects of probiotic supplementation on milk production, blood metabolite profile and enzyme activities of ewes during lactation. Ital J Anim Sci. 2018;18(1):134-9. doi: 10.1080/1828051x.2018.1496040.

  • 26.

    Witte VC, Krause GF, Bailey ME. A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. J Food Sci. 1970;35(5):582-5. doi: 10.1111/j.1365-2621.1970.tb04815.x.

  • 27.

    Grau R, Hamm R. [A simple method for the determination of water binding in muscles]. Die Naturwissenschaften. 1953;40(1):29-30. German. doi: 10.1007/bf00595734.

  • 28.

    Qureshi MA, Havenstein GB. A comparison of the immune performance of a 1991 commercial broiler with a 1957 randombred strain when fed "typical" 1957 and 1991 broiler diets. Poult Sci. 1994;73(12):1805-12. doi: 10.3382/ps.0731805. [PubMed: 7877936].

  • 29.

    Sakamoto K, Hirose H, Onizuka A, Hayashi M, Futamura N, Kawamura Y, et al. Quantitative study of changes in intestinal morphology and mucus gel on total parenteral nutrition in rats. J Surg Res. 2000;94(2):99-106. doi: 10.1006/jsre.2000.5937. [PubMed: 11104649].

  • 30.

    Mookiah S, Sieo CC, Ramasamy K, Abdullah N, Ho YW. Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J Sci Food Agric. 2014;94(2):341-8. doi: 10.1002/jsfa.6365. [PubMed: 24037967].

  • 31.

    SAS Institute. SAS User's Guide. SAS Institute; 2005. Report No.: Release 9.1.3.

  • 32.

    Duncan DB. Multiple range and multiple F tests. Biometrics. 1955;11(1):1. doi: 10.2307/3001478.

  • 33.

    Jiang ZY, Jiang SQ, Lin YC, Xi PB, Yu DQ, Wu TX. Effects of soybean isoflavone on growth performance, meat quality, and antioxidation in male broilers. Poult Sci. 2007;86(7):1356-62. doi: 10.1093/ps/86.7.1356. [PubMed: 17575182].

  • 34.

    Popova MP, Popov CS. Damage to subcellular structures evoked by lipid peroxidation. Z Naturforsch C. 2002;57(3-4):361-5. doi: 10.1515/znc-2002-3-427. [PubMed: 12064741].

  • 35.

    Amad AA, Manner K, Wendler KR, Neumann K, Zentek J. Effects of a phytogenic feed additive on growth performance and ileal nutrient digestibility in broiler chickens. Poult Sci. 2011;90(12):2811-6. doi: 10.3382/ps.2011-01515. [PubMed: 22080020].

  • 36.

    Alp M, Midilli M, Kocaba l  N, Y lmaz H, Turan N, Garg l  A, et al. The effects of dietary oregano essential oil on live performance, carcass yield, serum immunoglobulin G level, and oocyst count in broilers. J Appl Poultr Res. 2012;21(3):630-6. doi: 10.3382/japr.2012-00551.

  • 37.

    Amresh G, Reddy GD, Rao Ch V, Singh PN. Evaluation of anti-inflammatory activity of Cissampelos pareira root in rats. J Ethnopharmacol. 2007;110(3):526-31. doi: 10.1016/j.jep.2006.10.009. [PubMed: 17097249].

  • 38.

    Hajati H, Rezaei M. The application of prebiotics in poultry production. Int J Poultr Sci. 2010;9(3):298-304. doi: 10.3923/ijps.2010.298.304.

  • 39.

    Mohiti-Asli M, Ghanaatparast-Rashti M. Comparing the effects of a combined phytogenic feed additive with an individual essential oil of oregano on intestinal morphology and microflora in broilers. J Appl Anim Res. 2017;46(1):184-9. doi: 10.1080/09712119.2017.1284074.

  • 40.

    Viveros A, Chamorro S, Pizarro M, Arija I, Centeno C, Brenes A. Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poult Sci. 2011;90(3):566-78. doi: 10.3382/ps.2010-00889. [PubMed: 21325227].

  • COMMENTS

    LEAVE A COMMENT HERE: