Method Validation for the One-Month Stability Study of trans–Resveratrol in Human Plasma

AUTHORS

Milad Iranshahy 1 , 2 , Amir Hooshang Mohammadpour 3 , Mohammad Hassanzadeh-Khayyat 4 , Mehrdad Iranshahi 1 , 2 , *

1 Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran

2 Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran

3 Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran

4 Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran

How to Cite: Iranshahy M, Mohammadpour A H, Hassanzadeh-Khayyat M, Iranshahi M. Method Validation for the One-Month Stability Study of trans–Resveratrol in Human Plasma, Jundishapur J Nat Pharm Prod. 2013 ; 8(2):e94152. doi: 10.17795/jjnpp-9566.

ARTICLE INFORMATION

Jundishapur Journal of Natural Pharmaceutical Products: 8 (2); e94152
Published Online: May 04, 2013
Article Type: Research Article
Received: May 20, 2019
Accepted: January 30, 2013
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Abstract

Background: trans-Resveratrol (t-Res) is a natural phenolic compound with various biological activities such as antioxidant, anticancer, cancer chemopreventive, cardioprotective, and neuroprotective.

Objectives: There is no comprehensive study about the stability of t-Res in human plasma. With respect to extensive use of t-Res in clinical trials and pharmacokinetic studies, in this study, we aimed to measure the plasma stability of t-Res at different conditions of lighting, pH, and temperature, and in the presence of routine antioxidant agents (BHT and VIT C).

Materials and Methods: t-Res stability in human plasma was evaluated at different conditions of lighting, pH and temperature, and in the presence of routine antioxidant agents (BHT and VIT C), and quantitatively determined using HPLC-UV method at 306 nm.

Results: Our findings revealed that t-Res was quite stable in different temperatures ranging from -70 °C to 25 °C, and acidic pH conditions in plasma for a month, when protected from light, but it was unstable in alkali pH and in lighting conditions.

Conclusions: In lighting conditions, most of t-Res was isomerized to the other isomer cis-resveratrol. t-Res is unstable in alkali pH and when exposed to light. t-Res was quite stable at other conditions, and several freeze-thaw cycles did not have any effect on its stability.

Keywords

Resveratrol Stability Antioxidants

© 2013, Jundishapur Journal of Natural Pharmaceutical Products. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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